小肠粘膜下层在膀胱组织修复中生长因子释放的研究  

Bladder Regeneration by Small Intestinal Submucosa with Release of Exogenous Growth Factors

作　　者：林茂虎[1,2] 郁华亮[3] 苗芮[3] 秦万长[3] 贾宁[4] 李娟 

机构地区：[1]第四军医大学研究生院 [2]解放军总医院第一附属医院泌尿外科,北京市100037 [3]解放军总医院第一附属医院泌尿外科 [4]解放军总医院医院感染与疾病控制科 [5]二炮通信总站卫生队

出　　处：《中国康复理论与实践》2006年第7期578-580,F0003,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(No.30300349)。

摘　　要：目的了解小肠粘膜下层（SIS）在膀胱组织修复中外源性生长因子血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）释放。方法用ELISA法和MTT法体外测量冻干SIS在PBS孵育液中VEGF和bFGF的含量及对上皮细胞的增殖作用；用猪小肠粘膜下层行大鼠膀胱部分修复。术后在不同时间观察大鼠膀胱修复情况，并用组织学免疫组化方法观察VEGF和bFGF的表达，单纯大鼠粘膜及部分肌层缺损组作为对照。结果SIS在PBS孵育液中bFGF含量约为（121．8±2．683）ng／L，VEGF含量约为（93．8±3．033）ng／L，且对上皮细胞有促进增殖作用。移植的SIS上可见新生毛细血管和平滑肌肌束。实验组和对照组VEGF和bFGF在术后第1周均呈弱阳性表达，第2周以后实验组VEGF和bFGF表达逐渐增强，至第6周达到高峰，第8周VEGF和bFGF的表达逐渐减弱；而对照组在第1～10周VEGF和bFGF的表达均呈弱阳性，未见明显的表达高峰。结论SIS作为载体将外源性生长因子带人大鼠体内，在SIS逐渐降解的同时释放生长因子，刺激血管生成和宿主细胞的长人和分化。Objective To explore the release of exogenous growth factors from small intestinal submucosa （SIS） in bladder regeneration. Methods The release of vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） from SIS in vitro were evaluated by ELISA and MTT method. The defected bladder walls of rats in experimental group were repaired with porcine small intestinal submuscosa. Partial bladder mucosa and smooth muscle of the rats in control groups were destroyed. At regular intervals, the VEGF and bFGF expression were observed by histological and immunohistochemical methods. Results The concentration of bFGF and VEGF released in vitro from SIS in PBS solution were （121.8±2. 683） ng/L and （93.8±. 033） ng/L respectively, and showed proliferation of vascular endothelial cell. In the SIS framework, the capillary and smooth muscle were observed followed histological evaluation. The weak expression of VEGF and bFGF in both experimental and control groups were found in the first week. Since the second week the VEGF and bFGF expression in experimental group began to increase with a peak in the 6th week, and began to decrease after 8 weeks. In the control group, the weak VEGF and bFGF expression were shown during the observation. Conclusion SIS functions as a carrier for exogenous growth factors release in rat bladder regeneration.

关 键 词：小肠粘膜下层(SIS) 膀胱 修复 血管内皮生长因子(VEGF) 成纤维细胞生长因子(FGF) 大鼠 

分 类 号：R694[医药卫生—泌尿科学]