中华结缕草组织培养与植株再生  

Tissue culture and plant regeneration of Zoysia siasinica

作　　者：杜雪玲[1] 张振霞[2] 刘萍[3] 龚春水[3] 符义坤[4] 杨中艺[3] 

机构地区：[1]安徽淮北煤炭师范学院生物系,安徽淮北235000 [2]韩山师范学院生物系,广东潮州521041 [3]中山大学生命科学院,广东广州510275 [4]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《草业学报》2006年第4期88-93,共6页Acta Prataculturae Sinica

基　　金：国家转基因植物研究与产业化专项(J2002-B-006)资助

摘　　要：以中华结缕草成熟胚为外植体，通过胚性愈伤组织的发生进行植株再生。结果表明．中华结缕草成熟胚在MS基本培养基附加6～7mg／L2．4-D和0．05mg／L6-BA的出愈能力很强．但初生愈伤组织呈无定型棉絮状，不能再生成株。采用不同的糖类和固化剂以及一定比例的生长素与细胞分裂素的方法，对初生愈伤组织进行继代培养，诱导出致密颗粒状的、具有高度再生能力的胚性愈伤组织，此类胚性愈伤组织在最佳分化培养基MS＋1．0mg／LNAA＋1．0mg／LKT＋3．00mg／L6-BA上有较高再生频率．生根培养基采用1／2MS。A tissue culture system for plant regeneration of Zoysia siasinica Hance grass was developed. Using mature seeds as experimental material, explant callus was produced in the dark on MS containing 100 mg/L Linostial＋ 5 mg/L2,4-D＋ 0.05 mg/L6-BA, 30 mg/L sucrose and 3.4 g/L phytagel. The initial callus appeared sponge-like, soft, and irregular, and no plants could be regenerated from it. High regeneration ability embryogenie calluses were induced after subculturing with 2 mg/L ABA. The differentiation frequency of callus subcultured for 80 days was up to 20.83% in MS with 1.0 mg/L NAA,1.0 mg/L KT and 3.00 mg/L 6-BA MS. The rooting medium was 1/2 MS.

关 键 词：中华结缕草 愈伤组织 植株再生 

分 类 号：S688.4[农业科学—观赏园艺]