Application of Non-invasive Microsensing System to Simultaneously Measure Both H^＋ and O2 Fluxes Around the Pollen Tube  

作　　者：Yue Xu Tong Sun Li-Ping Yin 

机构地区：[1]College of Life Sciences, Capital Normal University, Beijing 100037, China [2]Younger USA Company, Cary, NC 27513, USA [3]Vibrating Probe Facility, Biology Department, University of Massachusetts at Amherst, MA 01003, USA

出　　处：《Journal of Integrative Plant Biology》2006年第7期823-831,共9页植物学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （30170552）, Beijing Natural Science Foundation （5042004）, Beijing Education Committee Science and Technology Development Project （KM200410028015） and Applicable Electronics Inc. （Forestdale, MA, USA）.

摘　　要：Various Ionic and molecular activities in the extraceUular environment are vital to plant cell physiological processes. A noninvasive microsensing system （NMS） based on either the scanning ion-selective electrode technique （SIET） or the scanning polarographlc electrode technique （SPET） is able to obtain information regarding the transportation of various Ions/molecules in Intact samples under normal physiological conditions. The two-probe simultaneous test system （2STS） Is an Integrated system composed of SIET, SPET, and a Xu-Kunkel sampling protocol. In the present study, 2STS was able to simultaneously measure fluxes of H^＋ and O2 of the Uly （Lillum Iongiflorum Thunb. cv. Ace） pollen tube while avoiding interference between the two probes. The results Indicate that the proton fluxes were effluxes, whereas the oxygen fluxes were Influxes, and they were closely correlated to each other surrounding the constitutive alkaline band region. Specifically, when the proton effluxes increased, the oxygen Influxes also increased. Therefore, the hypothesis of condensed active mitochondria existing in the alkalized area of the pollen tube proposed by Hepler＇s group is supported.Various Ionic and molecular activities in the extraceUular environment are vital to plant cell physiological processes. A noninvasive microsensing system （NMS） based on either the scanning ion-selective electrode technique （SIET） or the scanning polarographlc electrode technique （SPET） is able to obtain information regarding the transportation of various Ions/molecules in Intact samples under normal physiological conditions. The two-probe simultaneous test system （2STS） Is an Integrated system composed of SIET, SPET, and a Xu-Kunkel sampling protocol. In the present study, 2STS was able to simultaneously measure fluxes of H^＋ and O2 of the Uly （Lillum Iongiflorum Thunb. cv. Ace） pollen tube while avoiding interference between the two probes. The results Indicate that the proton fluxes were effluxes, whereas the oxygen fluxes were Influxes, and they were closely correlated to each other surrounding the constitutive alkaline band region. Specifically, when the proton effluxes increased, the oxygen Influxes also increased. Therefore, the hypothesis of condensed active mitochondria existing in the alkalized area of the pollen tube proposed by Hepler＇s group is supported.

关 键 词：non-invasive microsensing system （NMS） oxygen fluxes pollen tube proton fluxes scanning ion-selective electrode technique （SIET） scanning polarographic electrode technique （SPET） two-probe simultaneous test system （2STS）. 

分 类 号：Q944.42[生物学—植物学]