Effects of Exogenous Spermidine on PhotosystemⅡof Wheat Seedlings Under Water Stress  

作　　者：Hui-Guo Duan Shu Yuan Wen-Juan Liu De-Hui Xi Dong-Hong Qing Hou-Guo Liang Hong-Hui Lin 

机构地区：[1]Key Laboratory of Bio-resources and Eco-environment （Ministry of Education）, College of Life Sciences, Sichuan University, Chengdu 610064, China [2]Department of Chemistry and Life Sciences, Neijiang Teachers＇ College, Neijiang 641112, China

出　　处：《Journal of Integrative Plant Biology》2006年第8期920-927,共8页植物学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(30571119);the Sichuan Science and Technology Foundation(04ZQ026-036 and 05JY029-017);the Education Ministry Doctoral Foundation of China (20040610015);the Chengdu Science and Technology Foundation (05HJSW117)

摘　　要：The effects of exogenous spermidine （Spd） on lipid peroxidation, relative plasma membrane permeability, photosystem Ⅱ （PSII） gene expression and PSII photochemical activity in water-stressed wheat seedlings were investigated. The decrease in relative water content （RWC）, Chl content, and 2,6-dichlorophenol indophenol （DCIP） photoreduction of PSII, and increases in electrolyte leakage of plasma membranes and malonyldialdehyde （MDA） in water-stressed leaves was alleviated by Spd pretreatment. Furthermore, Western and Northern blot analysis showed that decreases in the PSll major proteins D1, D2 and LHCII and the transcripts of corresponding genes psbA, psbD and cab were also alleviated by Spd pretreatment under water stress. These results suggest that the application of exogenous Spd protects PSII against water stress at both the transcriptional level and the translational level, and allows PSII to retain a higher activity level during water stress. The protective role of Spd in the photosynthetic apparatus also is discussed.The effects of exogenous spermidine （Spd） on lipid peroxidation, relative plasma membrane permeability, photosystem Ⅱ （PSII） gene expression and PSII photochemical activity in water-stressed wheat seedlings were investigated. The decrease in relative water content （RWC）, Chl content, and 2,6-dichlorophenol indophenol （DCIP） photoreduction of PSII, and increases in electrolyte leakage of plasma membranes and malonyldialdehyde （MDA） in water-stressed leaves was alleviated by Spd pretreatment. Furthermore, Western and Northern blot analysis showed that decreases in the PSll major proteins D1, D2 and LHCII and the transcripts of corresponding genes psbA, psbD and cab were also alleviated by Spd pretreatment under water stress. These results suggest that the application of exogenous Spd protects PSII against water stress at both the transcriptional level and the translational level, and allows PSII to retain a higher activity level during water stress. The protective role of Spd in the photosynthetic apparatus also is discussed.

关 键 词：gene expression photosystem Ⅱ SPERMIDINE water stress wheat seedlings. 

分 类 号：Q949[生物学—植物学]