血管化组织工程骨修复牙槽嵴裂的实验研究  

An investigation of restoration of cleft alveolar with vascularised engineered bone

作　　者：欧新荣[1] 翦新春[1] 卢光琇 

机构地区：[1]中南大学湘雅医院口腔医学院颌面外科,湖南长沙410008 [2]国家人类干细胞工程技术研究中心

出　　处：《临床口腔医学杂志》2006年第8期465-468,共4页Journal of Clinical Stomatology

基　　金：湖南省自然科学基金资助项目(05JJ30171)

摘　　要：目的:探讨应用经血管内皮细胞生长因子(VEGF)转染的骨髓基质干细胞(BMSC)复合胶原蛋白海绵构建的血管化组织工程骨修复牙槽嵴裂的可行性。方法:①经股骨骨髓穿刺获得犬BMSC,经分离、培养和鉴定后,转染pAdMV-VEGF质粒,流式细胞仪、RT-PCR和免疫组化检测;转染细胞与蛋白胶原海绵混合培养72 h形成复合体。②将复合体植入犬牙槽嵴裂模型的骨缺损处,12周后处死动物,通过大体形态及X线、三维CT重建、组织学检查等方法评价修复牙槽嵴裂的效果。结果:实验组骨缺损完全愈合,骨髓腔通畅,工程骨高度及宽度与自体松质骨植入组无明显差别(P>0.05)。结论:由经VEGF转染的BMSC复合胶原蛋白海绵构建的组织工程骨可以较好地修复牙槽嵴裂,在修复后的形态、结构等方面均与自体松质骨修复相似,有望成为修复牙槽嵴裂的治疗方法。Objective：To investigate the feasibility of the restoration of cleft alveolar with vascularised engineered bone constructed by collagen combined with vascular endothelial growth factor （VEGF） gene transfected bone mesenchymal stem cells （BMSC）. Method：The BM~ were isolated by gradient density centrifugation with Percoll solution from dog bone marrow. After being cultured and VEGF165 gene transfected, detected by FCM,RT-PCR and IHC, BMSCs were seeded in collagen for 72 hours. The composites of BMSCs and collagen were implanted into the defect of cleft alveolar of dogs . After being fed for 12 weeks, those dogs were sacrificed. X-ray, three-dimensional CT and histological examination were used to observe the bone formation. Result：52% of BMSCs were transfected with VEGF165 plasmid. The defects of cleft alveolar healed at 12 weeks after being implant collagen and VEGF165 plasmid transfected BMSC composites and the forms of engineered bone resembled those implanted by autologous cancellous bone, Conclusion： The vascularised engineered bone can repair the defect of alveolar cleft effectively, it may be used in the clinical treatment of the restoration of alveolar cleft.

关 键 词：血管化 组织工程骨 血管内皮细胞生长因子 牙槽嵴裂 

分 类 号：Q813.1[生物学—生物工程]