地塞米松脂质体壳聚糖胶囊对大鼠结肠炎的药效学研究  

Effect of Chitosan Capsule Filled with Dexamethasone Liposome Lyophilized Powder on Colitis in Rats

作　　者：李国锋[1] 陈建海[1] 赖卓胜[2] 王春霞[1] 杨西晓[1] 许重远[1] 杨莉[1] 侯连兵[1] 

机构地区：[1]南方医科大学南方医院药学部,广州510515 [2]南方医科大学南方医院消化科,广州510515

出　　处：《中国药学杂志》2006年第15期1145-1149,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(39970784);广东省自然科学基金资助项目(020056);广东省医学科研基金资助项目(A2002371)

摘　　要：目的评价地塞米松(DSP)脂质体冻干剂壳聚糖胶囊对结肠炎大鼠的治疗作用。方法用2,4,6-三硝基苯磺酸钠(TNBS)诱导大鼠结肠炎,建立髓过氧化酶(MPO)、肿瘤坏死因子(TNF-α)、大鼠结肠质量与大鼠质量比值(C/B)和结肠损伤直观评分值4种反应结肠炎症程度指标的测定方法,观察口服各种DSP制剂对结肠炎症的治疗作用。结果用TNBS灌肠时可以成功地制备大鼠结肠炎症模型,灌肠后第5天时反映炎症的各种指标均达到最高值;各种DSP制剂对TNBS诱导的实验性大鼠结肠炎均有治疗效果,而DSP脂质体壳聚糖胶囊具有最好的治疗作用。结论DSP脂质体冻干剂壳聚糖胶囊较DSP粉剂壳聚糖胶囊和DSP溶液对结肠炎具有更好的局部治疗效果,值得进一步开发研究。OBJECTIVE To observe the effect of chitosan capsule filled with dexamethasone（DSP） liposome lyophilized powder on colitis in rats. METHODS The colitis in rat was induced with the coloclysis of 2, 4, 6-trinitrobenzene sulfonic acid（TNBS）. The myeloperioxidase （MPO）, tumor necrosis factor（TNF-α）, the ratio of rat colon weight/rat body weight（C/B） as well as the colon damage score（Score）, which were chosen as the index of colitis degree, were determined. These indexes were used to evaluate the phannacodynamic action of various DSP dosage forms after their oral administration. RESULTS The colitis was successfully induced with the coloclysis of TNBS and the inflammation was the most severe in the fifth day. All DSP preparations effectively relieved the colitis in rat, while DSP lyophilized liposome capsule showed optimum effect. CONCLUSION DSP lyophilized liposome chitosan capsule may be the most helpful in colitis therapy among all DSP dosage forms in the study.

关 键 词：地塞米松 壳聚糖胶囊 脂质体 结肠炎 药效学 

分 类 号：R965[医药卫生—药理学]