等容血液稀释和1，6－二磷酸果糖对兔缺血再灌注心肌的保护作用  

Protective effect of isovolumetric hemodilution and Fructose-1,6-diphosphate(FDP) on ischemic reperfused myocardium in rabbits

作　　者：汤宁[1] 汪丽娟[1] 吕杰胜[1] 赵焕芬 姜文霞[1] 鄢文海[1] 宋伟[1] 黄幼田[1] 王柏生[1] 岳鹤声 

机构地区：[1]河南医科大学病理生理学教研室,河南医科大学第一附属医院内科,河北省人民医院病理科,河南医科大学药学系

出　　处：《河南医科大学学报》1996年第4期21-24,共4页Journal of Henan Medical University

基　　金：河南省自然科学基金

摘　　要：心肌缺血４５ｍｉｎ，再灌注１８０ｍｉｎ复制兔急性心肌缺血再灌注模型。用亚硝酸盐法测定心肌ＳＯＤ活性，用硫代巴比妥酸比色法测定心肌ＭＤＡ含量。分别观察了等容血液稀释、１，６－二磷酸果糖（ＦＤＰ）以及二者联合应用对兔缺血再灌注心肌ＳＯＤ活性和ＭＤＡ含量的影响。实验共分４组：１组（对照组）；２组（稀释组）；３组（ＦＤＰ组）；４组（稀释＋ＦＤＰ组）。结果：①与１组缺血区心肌ＭＤＡ含量相比，２、３、４组均显著降低（Ｐ＜０．０５）；４组又明显低于２、３组（Ｐ＜０．０５）。②２、３、４组缺血区心肌ＳＯＤ活性均明显高于１组（Ｐ＜０．０５）；４组与２、３组相比又有所升高，但无显著差异。提示：等容血液稀释和ＦＤＰ均能通过保护ＳＯＤ活性及降低ＭＤＡ含量而保护缺血再灌注心肌；二者合用效果更加明显，表现出良好的协同效应。The purpose of the present study was to investigate the effects of hemodilution, FDP and the combined treatment of the two interventions on isc hemic reperfused myocardium.45 min coronary artery occlusion was followed by 180min reperfusion in order to reproduce acute myocardial ischemia-reperfusion models, which were divided into four groups : Group 1(controls); Group 2(Hemodilution); Group 3(FDP)and Group 4(Hemodilution+FDP).MDA production in myocardium was determined with TBA method and SOD activities with nitrite method. Results :①MDA contents in Group 2, 3 and 4 decreased significantly as compared with those in Group 1(P<0. 05), and those in Group 4 decreased further;②SOD activities in Group 2, 3 and 4 increased more significantly than those in Group 1(P<0.05).The results indicated that the effects of the combined treatment were much better than those of any one which was used alone.

关 键 词：心肌缺血 再灌注损伤 心肌保护 二磷酸果糖 

分 类 号：R541.4[医药卫生—心血管疾病] R363.21[医药卫生—内科学]