改良法深低温保存猪主动脉的组织学检查  

CRYOPRESERVATION OF SWINE AORTAE BY MODIFIED METHOD A HISTOLOGICAL STUDY

作　　者：孟凡会[1] 王吉秋[1] 王沛涛[1] 崔向东[1] 付秀艳[1] 于华英 

机构地区：[1]青岛医学院附属医院

出　　处：《青岛医学院学报》1996年第1期9-11,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省科委基金

摘　　要：①目的为配合临床移植，将Ｏ＇Ｂｒｉｅｎ法程序简化，以期达到同样的保存效果。②方法取１６段猪新鲜主动脉，其中８段用Ｏ＇Ｂｒｉｅｎ法，２４ｈ培养消毒后冷冻保存。冷冻保护剂为含有二甲基亚砜的Ｍ１９９液。８段采用改良法保存——即不进行２４ｈ培养消毒。新鲜标本、２４ｈ培养后及冷冻复温后分别取材行ＨＥ，Ｖｅｒｈｏｅｆ法染色行光镜及扫描电镜观察。③结果改良法深低温保存的主动脉壁大部分结构完整，与Ｏ＇Ｂｒｉｅｎ法相比无明显差异。④结论组织学检查结果表明改良法保存的主动脉效果良好，与传统的方法相比简单、实用。Objective To study the effect of modified OBrien method on swine aortae. Method 16 segments of fresh swine aortae were obtained. Eight of them were cultured and steri lized for 24 hours, and then cryopreserved by M199 which contains DMSO (OBrien method); the other eight segments were directly cryopreserved by M199 without being cultured and sterilized (modified method). Samples were obtained in fresh condition, after 24 hours culturing and sterilization, and after thawing. All specimens were observed under light microscope and scanning electron microscope. Result Most of the aortic architecture cryopreserved by modified method was good. There was no significant difference between the two methods. Conclusion The histological study showed that the modified OBrien method provided good effect. It was simpler and more practical than the standard OBrien method and could be widely used in clinical practice.

关 键 词：主动脉 组织学 低温保存 主动脉瓣移植 

分 类 号：R318.52[医药卫生—生物医学工程] R654.2[医药卫生—基础医学]