植物激素对砀山酥梨脱病毒苗增殖生长的影响  

Effects of Plant Hormones on Shoot-growth in the Course of Subculture Propagation of Virus-free Pyrus spp

作　　者：金青[1] 刘小阳[2] 蔡永萍[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]宿州学院化学与生物系,安徽宿州234000

出　　处：《激光生物学报》2006年第4期378-382,共5页Acta Laser Biology Sinica

基　　金：安徽省教育厅"十五"学术带头人基金项目(2002年度)

摘　　要：砀山酥梨脱病毒苗培养基中添加外源激素能通过调节内源激素的含量,从而控制脱病毒苗的增殖和生长。苄基腺嘌呤(benzyladen ine,BA)处理可提高脱病毒苗内源玉米素核苷(zeatin riboside,ZR)含量,而脱病毒苗的有效增殖芽数与IAA/ZR比值呈显著负相关;1萘-乙酸(1-naphthalene acetic ac id,NAA)处理可显著提高内源吲哚乙酸(indole acetic ac id,IAA)含量,较高的内源IAA含量有利于芽梢的生长;继代组培苗体内含有一定量的内源赤霉素(G iberllic Ac id,GA1+3),适量的外源GA3处理,可提高内源GA1+3含量而显著降低脱落酸(absc isic ac id,ABA)含量,促进芽梢的伸长和叶面积的扩大。Exogenous hormones in medium for propogation of Pyrus spp plantlet caused endogenous hormones changed and led to subculture plantlet proliferating and growing. Content of ZR increased and there was a significant negative correlation between the rate of IAA/ZR and effective proliferation shoots in BA treated. A significant increase in IAA level when NAA treated, which brought a greater shoot growth. Content of GA1 ＋3 was found in initial subculture plantlet, which increased with suitable GA3 treatment, meanwhile the content of ABA dropped significantly and thus promoting the shoot growth and size enlargement of leaves.

关 键 词：砀山酥梨 脱病毒苗 外源激素 内源激素 继代繁殖 

分 类 号：Q945[生物学—植物学] S661.2[农业科学—果树学]