模拟高原缺氧大鼠骨髓细胞EPOR亲和力及数量的测定  

Effects of simulated high altitude on affinity and quantity of erythropoietin receptor in rats' bone marrow cells

作　　者：吴洲[1] 宋玲[1] 高钰琪[1] 孙秉庸[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理教研室,重庆400038

出　　处：《西南国防医药》2006年第4期360-362,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金重点资助项目(NO:39730190)

摘　　要：目的:探讨促红细胞生成素受体(EPOR)在高原缺氧诱导的红细胞增多发生机制中的作用。方法:Wistar大鼠随机分为常氧对照组和模拟高原缺氧5 d1、5 d、30 d组共4组。利用同位素技术观察骨髓细胞EPOR亲和力和数量的改变。结果:和对照组大鼠相比,缺氧5 d组大鼠骨髓细胞EPOR亲和力显著升高,而缺氧15 d组和30 d组大鼠的骨髓细胞EPOR亲和力反而是显著降低(P<0.01)。单个骨髓细胞表面EPOR的数量在整个缺氧过程中呈现显著增高,并且缺氧30 d组显著高于缺氧5 d组。结论;缺氧诱导的EPOR亲和力与数量的改变在缺氧早期红细胞增多过程中起重要作用。缺氧后期可能有其他因子参与红细胞增多的调节。Objective：To investigate the roles of erythropoietin receptor（EPOR） in the mechanism of erythrocytosis at high altitude. Methods：Adult Wistar rats were randomly divided into 4 groups： normal group（normoxia） and other 3 groups that were exposed to simulated high altitude（above seal level 5 000 m） for 5 days, 15 days and 30 days, respectively, 125I - EPO was used to demonstrate expression of saturable high affinity binding sites for EPO in rat bone marrow cells. Results-Compared with the normoxia group, the affinity of EPOR in rat bone marrow cells increased in 5 - day groups, and declined in 15 - day and 30 - day groups（P〈0. 01）. Binding sites per cell showed an increased trend along with hypoxic process Conclusion：The increases of affinity and quantity of EPOR in rat bone marrow cells induced by hypoxia plays an important role in the development of erythrocytosis at first, but at later stage other factors maybe play more important role in the development of erythrocytosis.

关 键 词：高原缺氧 亲和力 促红细胞生成素受体 

分 类 号：R551[医药卫生—血液循环系统疾病]