异甜菊醇抗麻醉大鼠心肌缺血复灌损伤的作用机制  

The mechanism of the cardioprotective effect of isostevioside in anesthetized rats

作　　者：李永芳[1] 许德义[2] 

机构地区：[1]青海大学医学院药理教研室,青海西宁810001 [2]东南大学医学院药理教研室,江苏南京210009

出　　处：《华西药学杂志》2006年第4期331-334,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究双萜类化合物异甜菊醇抗心肌缺血复灌损伤作用与线粒体ATP敏感性钾通道(m itoKATP)开放的关系。方法用经典的大鼠冠状动脉结扎30 m in,再通90 m in,形成心肌缺血复灌模型。造模前iv异甜菊醇1.0 m.gkg-1和/或选择性m itoKATP关闭剂5-羟基癸酸(5-HD)10 m.gkg-1预处理,以血流动力学、心肌梗死面积、血清乳酸脱氢酶和肌酸激酶的活性、心肌丙二醛含量、SOD、GSH-PX活性和心肌组织病理学检查,评价异甜菊醇抗心肌缺血复灌损伤的作用与m itoKATP的关系。结果异甜菊醇具有改善大鼠缺血复灌引起的心功能和组织损伤,降低心肌活性氧含量,但此作用可被预先给予的5-HD减弱。结论异甜菊醇抗心肌缺血复灌损伤的作用可能与m itoKATP的开放和活性氧减少有关。OBJECTIVE To study on the relationship between the cardioproteetive effect of isostevioside （ ST - 1 ） and mitochondrial ATP - sensitive potassium channels（mitoKATP） in vivo. METHODS The hemodynamics changes were determined during isehemia - reperfusion. The measures of myocardial area suffered from ischemia - reperfusion injury, activity of the serum LDH and CK, the SOD, GSH - PX activities and content of MDA of myocardium homogenate were determined at the end of the experiment. Myocardial structure was examined by microscope. RESULTS In 5 - HD ＋ ST - 1 group, the values of hemodynamics, the activities of SOD and GSH - PX were significantly less than those in ST - 1 pretreated group. Myocardial infarct size, the activities of serum LDH and CK, the content of MDA in myocardium were higher than those in ST - 1 pretreated group. The myocardial microstructures were worse than those in ST - 1 pretreated group, but each index was better than those in IR group. CONCLUSION The result is suggested that the cardioproteetive effect of ST- 1 against ischemia- reperfusion injury may be related to mitochondrial ATP- sensitive channels（mitoKATP） and reactive oxygen species.

关 键 词：异甜菊醇 心肌缺血 复灌损伤 线粒体ATP敏感性钾通道 活性氧 

分 类 号：R965[医药卫生—药理学]