不同PEG含量对mPEG-PLGA-mPEG纳米粒血浆蛋白吸附和体外细胞摄取的影响  

Influence of different PEG contents on the phagocytic uptake and plasma protein adsorption of mPEG-PLGA-mPEG nanoparticles

作　　者：全灵东[1] 徐雄良[1] 符垚[1] 孙逊[1] 龚涛[1] 张志荣[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2006年第4期348-350,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金重点项目资助课题(批准号:30430770)

摘　　要：目的考察不同PEG含量对mPEG—PLGA—mPEG（PELGE）纳米粒血浆蛋白吸附和巨噬细胞吞噬的影响。方法利用SDS—PAGE电泳技术，分析不同纳米粒的血浆蛋白吸附；以小鼠巨噬细胞RAW264．7为模型，采用化学发光法分析不同纳米粒的吞噬情况。结果与结论PEG含量为5％-10％时，纳米粒吸附最少的血浆蛋白，而且被巨噬细胞吞噬也最少。由此，可设计出适合于静脉注射的、可生物降解的、长循环的药物载体。OBJECTIVE To prepared monomethoxy （ polyethylene glycol ） - poly （ D, L - lactic - co - glycolic acid） - monomethoxy （polyethylene glycol） （mPEG -PLGE -mPET, PELGE ） nanoparticles and study the influence of PEG content on the competitive plasma protein adsorption and particle uptake by RAW264.7. METHODS Plasma protein adsorption onto PELGE nanoparticles was analysed through SDS - PAGE electrophoresis. And chemiluminescence, which does not need labelling, was used to follow the the uptake of various PEG - coated nanoparticles with the phagocytic cells. RESULTS AND CONCLUSION A PEG content between 5% - 10% was determined as a threshold value for optimal protein resistance and to avoid the uptake by RAW264.7 cells. When increasing the PEG content in the nanoparticles above 10% no further reduction was achieved. These results could be useful in the design of long circulating intravenously injectable biodegradable drug carries endowed with protein resistance properties and low phagocytic uptake.

关 键 词：纳米粒 血浆蛋白 细胞吞噬 RAW264.7 

分 类 号：R945[医药卫生—微生物与生化药学]