槲皮素、三七总皂甙对动脉粥样硬化大鼠主动脉PDGF-B链mRNA表达的影响  

Effect of Quercetin and Panax Notoginseng Saponins on Expression of Platelet-derived Growth Factor B Chain mRNA in Rat's Aorta

作　　者：李霞[1] 李丽[2] 

机构地区：[1]山西中医学院,030024 [2]山西医科大学第二医院

出　　处：《中西医结合心脑血管病杂志》2006年第8期696-698,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的探讨槲皮素(Que)、三七总皂甙(tPNS)及二者不同比例的益气活血组方对实验性动脉粥样硬化(AS)大鼠主动脉血小板衍生生长因子B链(PDGF-B)mRNA表达的影响,以阐明其治疗AS的作用机制。方法以高脂饲料复制AS大鼠模型,造模成功后分别以Que、tPNS及二者2∶1、3∶1组方灌胃干预4周后,用反转录聚合酶链反应(RT-PCR)方法测定主动脉PDGF-BmRNA的表达。结果各干预组PDGF-BmRNA的表达与模型组比较,均有统计学意义(P<0.01),其中以3∶1组治疗效果最佳,而3∶1组与正常对照组、模型组、川芎嗪对照组的PDGF-BmRNA的表达相比,有统计学意义(P<0.01)。结论Que、tPNS及二者2∶1、3∶1益气活血组方都可以下调主动脉PDGF-BmRNA的表达。Objective To investigate the effect of quercetin and total panax notoginseng saponins and its two different proportional combinations on expression of platelet - derived growth factor B chain mRNA in aorta of rats. Methods Wistar rats were fed with high fatty diet for 12 weeks. Then quercetin, total panax notoginseng saponins or 2 ： 1,3 ： 1 of them were fed for rats in different groups for 4 weeks by gastrogavage respectively. Finally, the expression of platelet - derived growth factor B chain （PDGF- B） mRNA in atherosclerotic aorta of rats was detected by reverse transcription polymerase chain reaction（RT- PCR）. Results Comparing with model group, the expression of PDGF- B mlCNA in four therapy groups were all decreased significantly. Comparing with control group, model group and therapy group, the expression of PDGF - B mRNA in 3 ： 1 therapy group was reduced significantly. Conclusion Quercetin, total panax notoginseng saponins and combinations of them oould down- regulate the expression of PDGF- B mRNA. They might be played a role in anti - atherosclerosis through down - regulating the expression of PDGF - BmRNA.

关 键 词：槲皮素 三七总皂甙 血小板衍生生长因子 动脉粥样硬化 益气活血 

分 类 号：R285.5[医药卫生—中药学]