家犬脑挫裂伤^1H—MR波谱研究  

^1 H-MR spectroscopy of dog＇s brain contusion and laceration

作　　者：王学建[1] 张体江[2] 余晖[1] 沈桂权[1] 魏渝清[1] 李东方[1] 史黔华[1] 向志华[1] 

机构地区：[1]贵阳医学院附属医院放射科,贵州550004 [2]遵义医学院附属医院放射科

出　　处：《中华放射学杂志》2006年第8期875-878,共4页Chinese Journal of Radiology

摘　　要：目的探讨家犬脑挫裂伤MR波谱(MRS)表现及其应用价值。方法家犬10只,200 g砝码1．3 m高以自由坠落方式复制脑挫裂伤动物模型,分6个时间点(1 h、24 h、72 h、5 d、8 d和14 d)行常规MR及MRS检查。各时段检查结束后处死家犬,取挫裂伤处脑组织行病理检查。结果伤后1～24 h,N-乙酰天冬胺酸/肌酸(NAA/Cr)、胆碱类化合物(Cho)/Cr及NAA/Cho下降,NAA/Cr分别为0．843±0．214,0．862±0．204,对侧1．069±0．284,1．048±0．232,t=-7．227,-6．718：Cho/Cr 1．181±0．224,1．243±0．134,对侧1．415±0．305,1．455±0．159,t=-4．332,-4．489：NAA/Cho 0．701±0．147,0．536±0．136,对侧0．832±0．245,0．613±0．165,t=-2．652,-2．665(P值均＜0．05)；病理示挫伤处见局部点状出血、灶性坏死、神经轴突肿胀、小胶质细胞轻度增生。伤后72 h至5 d,NAA/Cr开始升高,Cho/Cr于5 d时为1．517±0．197,高于对侧,对侧为1．387±0．214(t=3．758。P＜0．05)；镜下示炎性细胞浸润明显,血管周围炎、肉芽及纤维瘢痕形成。伤后8～14 d,NAA/Cr与对侧相比差异无统计学意义(0．895±0．105,0．875±0．153,对侧0．989±0．169,0．990±0．173．t= -2．909,-2．471；P值均＞0．05),Cho/Cr高于对侧(1．457±0．168,1．572±0．374,对侧1．334±0．174,1．366±0．352,t=7．312,3．201；P值均＜0．05)；病理示炎性反应及胶质增生更加显著,14 d时肉芽肿形成。各时段均未见异常乳酸峰及脂质峰。结论MRS能无创性检测脑挫裂伤后神经元受损及修复情况,了解其脑组织生化代谢改变,反映损伤的程度,为早期治疗和预后的评价提供理论依据。Objective To investigate proton magnetic resonance spectroscopy （^1H-MRS） findings and value on dog＇s brain contusion and laceration. Methods Models of focal brain contusion and laceration in 10 dogs were established through hitting on the right frontal-parietal lobe with a freely drop of 200g weight at 1.3 m height. Serial examinations （ 1 h, 24 h, 72 h, 5 day, 8 day and 14 day after trauma） were performed with conventional MRI and ^1H-MRS. NAA/Cr, Cho/Cr and NAA/Cho rates were analyzed with GE system 1.5 T scanner and relative software. After examination, all dogs were executed to death. Pathological study was performed at local brain contusion. Results 1 h and 24 h-post trauma, NAA/Cr, Cho/Cr,NAA/Cho were significantly reduced （ NAA/Cr 0. 843 ± 0. 214,0. 862 ± 0. 204, contralateral ones 1. 069 ±0. 284,1. 048 ±0. 232,t = -7. 227, -6. 718,Cho/Cr 1. 181 ±0. 224,1. 243 ±0. 134,contralateral 1.415±0.305,1.455 ±0.159, t = -4.332, -4.489, NAA/Cho 0.701 ±0.147,0.536 ± 0.136, contralateral 0. 832 ± 0. 245,0. 613 ± 0. 165, t = - 2. 652, - 2. 665. P 〈 0. 05 ）. Microscopy showed focal petechial hemorrhage and necrosis, neuron loss, neuraxonal swelling and small glial cell slightly hyperplasia. Five day post trauma. , Cho/Cr was significantly elevated in comparison with contralateral ones （ 1. 517 ± 0. 197, contralateral 1. 387 ± 0. 214, t = 3. 758. P 〈 0. 05 ）. Pathologically, inflammatory was obvious, peri-angiitis, granula tissue and fibrosis were seen. 8-14 day later, NAA/Cr was not significantly reduced （0. 895 ± 0. 105,0. 875 ± 0. 153, contralateral 0. 989 ± 0. 169,0. 990 ± 0. 173, t = - 2. 909, -2. 471. P 〉 0. 05 ）, Cho/Cr was significantly increased （ 1. 457 ± 0. 168, 1. 572 ± 0. 374, contralaterl 1. 334 ± 0. 174,1. 366 ± 0. 352, t = 7.312,3. 201. P 〈 0. 05 ） Inflammatory and glial hyperplasia was more significant, granuloma were seen. Lipid and Lac peak were not seen at all stages. Conclusion MRS could be a methods to monitor neuron injury and repair, and dyn

关 键 词：颅脑创伤 磁共振成像 磁共振波谱学 动物 实验 

分 类 号：R445.2[医药卫生—影像医学与核医学]