一份条斑和颖花异常水稻双突变体的形态特征和细胞学观察  

MORPHOLOGICAL AND ANATOMICAL CHARACTERIZATION OF A STRIPE MUTANT WITH ABNORMAL FLORAL ORGANS IN RICE

作　　者：陈德西[1,2] 马炳田[1,2] 王玉平[1,2] 李仕贵[1,2] 郝铭[1,2] 

机构地区：[1]四川农业大学水稻研究所 [2]作物基因资源与遗传改良教育部重点实验室,四川农业大学,雅安625014

出　　处：《分子细胞生物学报》2006年第4期339-349,共11页Journal of Molecular Cell Biology

基　　金：教育部优秀博士论文基金(No:200054);教育部新世纪优秀人才支持计划资助;编号:NCET-04-0907;教育部创新团队发展计划资助;编号:IRT0453~~

摘　　要：在水稻遗传转化过程中发现一个不含外源基因的条斑和颖花异常的双突变体。该突变体的茎、叶、穗出现条斑。在分蘖盛期,一些叶片开始分岔或卷曲;花器官数目增多,表现为多内外稃,叶片状浆片,或浆片增大,雌雄蕊增多,颖花开裂。透射电镜对叶片白色组织细胞超微结构观察,发现细胞壁内陷,质体结构异常,不能发育出正常叶绿体所具有的片层和类囊体。叶绿素总含量和净光合速率明显低于野生型。突变体绿色组织部分中的细胞生长正常,但细胞较大。利用扫描电镜对花器官形态发生过程进行观察,雄蕊原基发育严重不同步,原基大小也不一样;心皮原基较小。A rice double mutant was derived from the transgenic process,but it does not carry the alien gene. The mutant showed white stripe on stem,leaf and spikelet. In some growing stage,the leaf started to produce fork or curliness. The floret number increased,showing multilemma/palea,palea-like or lemma-like lodicules or enlarged lodicules,additional pistil and stamen and the spited floret. With observation of cell ultra structure using electron microscope,the white tissue showed concaved cell wall and abnormal plastid which could not develop normal lamellae and thylakoid. The contents of chlorophyll and net photosynthesis rate in the mutant were obviously lower than those in the wild type. The cells in green sectors grow normally with the exception of the bigger cell volume. The morphogenesis of floral organ was observed by using the scanning electron microscopy （SEM）. Results showed that the stamen development was not synchronal and the sizes of stamen primordium were different in mutant,and the carpel was smaller than that of wild type.

关 键 词：水稻 条斑 叶绿体 花器官数目 细胞学观察 

分 类 号：Q943[生物学—植物学]