大鼠纹状体内移植神经干细胞的迁移分化行为  

THE MIGRATION AND DIFFERENTIATION OF SUBVENTRICULAR ZONE NEURAL STEM CELLS TRANSPLANTED INTO THE ADULT STRIATUM

作　　者：武孔彦[1] 马骥[1] 叶亦嫘[1] 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《分子细胞生物学报》2006年第4期357-364,共8页Journal of Molecular Cell Biology

基　　金：2004年度华东师范大学研究生科研基金支持

摘　　要：本文分离培养胎鼠脑室下带区(SVZ)神经干细胞,经含绿色荧光蛋白基因(GFP)的2型重组腺相关病毒感染,获得具有GFP标记的神经干细胞。标记后的细胞移植到成年SD大鼠纹状体内。分别在移植后45天、90天、120天时,取移植大鼠全脑进行矢状连续冰冻切片观察。结果显示,在各时间段,移植位点始终能检测到标记细胞,但有相当数量的细胞远离移植位点向周围迁移。移植后45天,细胞迁移出现明显的方向性、迁移细胞成链式排列。移植后120天,明显观察到两条迁移路线:一条沿弧形路线向背后侧迁移到达胼胝体下缘;另一条向腹后侧迁移到达黑质,并有细胞绕过或穿过黑质到达大脑底端。免疫组织化学分析显示,迁移细胞呈现β-tubulinⅢ阳性。Adeno-associated virus vectors （type2） containing the marker gene--green fluorescent protein （AAV2-GFP） were used to transduce subventricular zone neural stem cells （NSCs）. NSCs labelled by gene product GFP were transplanted into adult Sprague-Dawley rat striatum. The animals were allowed to survive for 45days,90days and 120days before they were perfused. All the fixed brains were serially sectioned in the saggital plane,at the sickness of 30μm with a cryostat microtome. These results showed that, at all stages,GFP labelled NSCs were seen in the injection sites,and a lot of them dispersed in the host brains. GFP labelled NSCs showed directional migration 45 days following transplantation. 120 days after transplantation,a group of GFP labelled NSCs migrated dorsally and posteriorly, reached corpus callosum; another group of transplanted NSCs migrated ventrally and posteriorly,reached substantia nigra, moreover,a number of these cells migrated through or over the substantia nigra and reached the more ventral boundary of the substantia nigra. The migrating NSCs were in chains,GFP labelled cells in substantia nigra were β-tubulinⅢ immunoreactive.

关 键 词：神经干细胞 腺相关病毒 绿色荧光蛋白 移植 迁移 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]