钙调蛋白在青少年特发性脊柱侧凸患者关节突骨组织中的表达  

Expression of calmodulin in the articular process of vertebrae of adolescent idiopathic scoliosis patients

作　　者：邱贵兴[1] 李婧[1] 刘勇[1] 吴志宏[1] 赵振国[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院骨科,100730

出　　处：《中华医学杂志》2006年第29期2017-2020,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目(30271312)

摘　　要：目的研究青少年特发性脊柱侧凸(MS)患者关节突骨质中钙调蛋白表达的差异。方法研究对象分为AIS组和先天性脊柱侧凸(CS)组,收集MS组上端椎和顶椎以及CS组融合范围内上端椎的凸凹侧下关节突。使用免疫组化及原位杂交在蛋白质和mRNA两个水平观察钙调蛋白的表达。使用Image-Pro Plus 5．1真彩色病理图像分析系统进行图像分析,对面密度结果进行统计学分析。结果钙调蛋白在关节突的骨细胞中表达,蛋白质和mRNA两个水平的表达一致:在凸凹侧关节突之间没有显著差异,顶椎同端椎之间没有显著差异(免疫组化结果分别是0．001 898和0．001 454,原位杂交结果分别是0．002 034和0．001 767),MS组明显低于CS组(免疫组化结果分别是0．001 454和0．005 346,原位杂交结果分别是0．001 767和0．007 621)(P<0．05)。结论钙调蛋白在AIS患者关节突骨质中的表达异常提示它可能通过直接调控骨质的代谢生长而影响AIS的发生发展。Objective To investigate the expression of calmodulin in the articular process of vertebrae of adolescent idiopathic scoliosis （AIS） patients and congenital scoliosis （CS） patients and its possible effects on the pathogenesis of AIS. Methods Nine AIS patients aged 13 - 17, and 10 patients of CS, aged 11 - 18, underwent orthopedic surgery. Specimens of the inferior articular process from the apical vertebrae and upper end vertebrae discarded during operation were collected from the AIS patients and specimens of the. Inferior articular process from the upper end vertebrae wee collected from the CS patients. Immunohistochemistry was used to detect the protein expression of calmodulin, and in situ hybridization was adopted to examine the mRNA expression of calmodulin. Results Calmodulin were expressed in the bone cells. There was no difference between the expression of calmodulin at the concave side and convex side, or between the apical vertebrae and end vertebrae. The expression of calmodulin of the AIS group was significant lower than that of the CS group （ P 〈 0. 05 ）. The result of immunohistochemistry and that of in situ hybridization corresponded with each other. Conclusion The expression of calmodulin in the articular process of vertebrae of AIS patients is low, suggesting that it plays a role in the development and progression of AIS.

关 键 词：脊柱侧凸 青少年 椎关节突关节 钙调蛋白 

分 类 号：R682[医药卫生—骨科学]