丝素导管修复面神经缺损的实验研究  

Experimental study on facial nerve regeneration by porous silk fibroin conduit

作　　者：陆艳[1] 迟放鲁[2] 赵霞[1] 邵正中[3] 曹正兵[3] 

机构地区：[1]复旦大学附属华山医院耳鼻咽喉头颈外科,上海200040 [2]复旦大学附属眼耳鼻喉科医院耳鼻咽喉头颈外科 [3]复旦大学高分子系

出　　处：《中华耳鼻咽喉头颈外科杂志》2006年第8期603-606,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家十五科技攻关计划(2004BA720A18-01);上海市卫生局科技发展基金项目(024075)

摘　　要：目的探讨多孔丝素材料用于面神经缺损修复的可能。方法以多孔丝素导管修复SD大鼠面神经5 mm缺损,设壳聚糖导管为对照。术后4、6、8周行电生理学检查,2、4、6、8周取材HE及甲苯胺蓝染色组织学检查,以了解神经再生及功能恢复情况。结果随时间推移,大鼠神经得以成功再生,8周时多孔丝素导管组的再生神经动作电位平均振幅已达健侧的24．9％±5．7％(x±s,以下同),与壳聚糖导管组(13．7％±3．4％)比较差异无统计学意义(P=1．125);8周时丝素导管组有髓神经纤维平均计数(62．5±6．3)与壳聚糖导管组(55．3±8．4)比较差异有统计学意义(P= 0．016)。结论多孔丝素导管对面神经缺损能起到一定作用,有可能促进和引导神经再生。Objective To evaluate the feasibility of using polyporous silk fibroin as a kind of novel material for facial nerve regeneration. Methods The porous silk fibroin conduit was used in the reconstruction of a 5 mm facial nerve gap of SD rat. Chitosan conduit was taken as control group. General observation, electrophysilogical study, histological study and image analysis were performed 2, 4, 6 and 8 weeks postoperatively. Results The facial nerve of SD rat regenerated successfully as time passed through. Mean CAP percentage of regenerated nerve in SF conduit was 24. 94% ± 5.73% 8 weeks postoperatively, which had no statistical significance with that of chitosan conduit group（P = 1. 125）. And the average number of myelinated myelinated nerve fibers in SF conduit was 62.5 ± 6. 3, which had statistical significance with that in chitosan conduit group （ P = 0. 016）. Conclusions The porous silk fibroin conduit could effectively repair facial nerve defect and improve peripheral nerve functional recovery.

关 键 词：面神经损伤 神经再生 丝素 

分 类 号：R651.3[医药卫生—外科学] R651.2[医药卫生—临床医学]