红景天对慢性高原心脏病大鼠心肺组织中内皮抑素和血管内皮细胞生长因子受体表达的影响  

THE INFLUENCE OF RHODILA ON THE EXPRESSION OF ENDOSTATIN AND VEGFR IN HEARTS AND LUNGS OF RATS WITH CHRONIC ALTIPLANO CARDIOPATHY

作　　者：隋岫兰[1] 陈荣华[2] 杨锋[3] 闫慧[3] 郑俊[1] 汪林勇[1] 噶布[1] 张世馥[3] 

机构地区：[1]西藏自治区第二人民医院,拉萨850002 [2]西藏自治区第一人民医院,拉萨850000 [3]中国医学科学院基础医学研究所,北京100005

出　　处：《解剖学报》2006年第4期436-439,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30160029)

摘　　要：目的探讨内皮抑素和VEGF受体与慢性高原心脏病发病的关系以及红景天治疗高原心脏病的机理。方法建立慢性高原心脏病大鼠模型,分为对照组、吸烟运动刺激组、拟高原心脏病组和治疗组。利用免疫荧光组织化学和半定量RT-PCR方法检测内皮抑素和VEGF受体的表达水平。结果刺激组心肺组织与对照组相比,VEGF受体表达水平升高,而内皮抑素下降。治疗组与拟高原心脏病组相比VEGF受体表达水平降低,而内皮抑素升高。结论慢性高原心脏病发病与内皮细胞损伤密切相关,红景天可以减少内皮细胞损伤。Objective To investigate the relationship between the expression of endostatin, VEGFR and chronic altiplano cardiopathy , and the mechanism of the curative effect of rhodiola, on treat altiplano cardiopathy. Methods The rat model with chronic altiplano cardiopathy was created. The experimental rats were divided into four groups： control group; smoking and running stimulated group; altiplano cardiopathy group and rhodiola treated group. The expression levels of endostatin and VEGFR were determined by the methods of immunohistochemistry and semi-quantitative RT-PCR. Results The expression levels of VEGFR in hearts and lungs of the stimulated groups were found higher than that of the control group, while endostatin level was contrary. The expression level of VEGFR in hearts and lungs of the rhodiola treated group was lower than that of the altiplano cardiopathy group, while endostatin was higher. Conclusion The pathogenesis of chronic altiplano cardiopathy seems to be closely associated with epithelial lesion. The curative effect of rhodila to this disease may be exerted by reducing the damage of epithelial ceils.

关 键 词：高原心脏病 血管内皮细胞生长因子 内皮抑素 红景天 免疫荧光组织化学 半定量RT-PCR 大鼠 

分 类 号：R555[医药卫生—血液循环系统疾病]