雌激素对培养新生大鼠心肌细胞凋亡的影响  

EFFECT OF ESTROGEN ON NOREPINEPHRINE-INDUCED APOPTOSIS IN CULTURED NEONATAL RAT CARDIOMYOCYTES

作　　者：朱艳霞[1] 田宗文[1] 杨晓宁[1] 王乔[1] 宋健[1] 

机构地区：[1]武汉大学医学院人体解剖与组织胚胎学系,武汉430071

出　　处：《解剖学报》2006年第4期444-449,共6页Acta Anatomica Sinica

基　　金：湖北省卫生厅重点资助项目(WJ01552)

摘　　要：目的探讨17β雌二醇(E2)对去甲肾上腺素(NE)诱导的培养心肌细胞凋亡的影响及其可能机制。方法培养的新生大鼠心肌细胞分别给予NE(50μmol/L)、E2(10 nmol/L)、NE(50μmol/L)+E2(10 nmol/L)作用48 h,倒置相差显微镜和透射电镜观察心肌细胞形态结构的变化;DNA ladder和流式细胞术对心肌细胞凋亡情况进行定性和定量分析;免疫荧光细胞化学技术及半定量分析心肌细胞促凋亡基因c-fos的蛋白表达。结果E2抑制NE诱导的心肌细胞凋亡的特征性形态学改变如细胞萎缩,核染色质浓缩边集,线粒体肿胀,出现凋亡小体等;使凋亡心肌细胞特异性的DNA梯状条带消失;降低心肌细胞凋亡率并减弱凋亡心肌细胞内c-fos蛋白的表达。结论17β雌二醇可抑制去甲肾上腺素所诱导的心肌细胞凋亡,其机制可能与促凋亡基因c-fos的表达有关。Objective To study the effect of 17β-estradial （E2） on apoptosis of cardiomyocytes induced by norepinephrine （NE） in vitro and its mechanism. Methods The cultured neonatal rat cardiomyocytes were treated with NE（50μmol/L）, E2 （10 nmol/L） or NE（ 50μmol/L） ＋ E2 （10 nmol/L） respectively in serum-free DMEM for 48 h. The morphological changes of myocytes were studied by phase-microscopy and transmission electron microscopy; apoptosis was identified by DNA laddering and apoptotic rate was assayed by flow cytometer; c-fos protein in cardiomyocytes was detected using semiquantitative immunofluorescence cytochemistry technique. Results 17β-estradiol inhibited the morphological changes of apoptotic cardiomyocytes induced by norepinephine such as cell atrophy, condensed chromatin clumps against the nuclear envelope associated with swelling of mitochondria and presentation of apoptotic body, DNA laddering disappeared, the apoptotic rate decreased and the expression of c-fos protein inhibited in cardiomyocytes. Conclusion 17β-estradiol can protect cultured cardiomyocytes from apoptosis induced by norepinephrine, its mechanism might associate with suppression of c-fos protein expression.

关 键 词：17Β雌二醇 去甲肾上腺素 凋亡 C-FOS 心肌细胞 流式细胞术 电子显微镜 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]