性激素对小鼠卵巢囊淋巴孔调控及淋巴吸收的影响  

EFFECT OF EXOGENOUS HORMONES ON LYMPHATIC STOMATA IN OVARIAN BURSA AND LYMPHATIC DRAINAGE IN MICE

作　　者：高赟[1] 李继承[1] 

机构地区：[1]浙江大学细胞生物学研究所,杭州310031

出　　处：《解剖学报》2006年第4期463-467,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30470910)

摘　　要：目的研究性激素对小鼠卵巢囊淋巴孔的作用及淋巴吸收的影响,并通过卵巢囊内层间皮细胞核内雌激素受体对性激素调控淋巴孔的开放加以探讨。方法应用示踪剂测定淋巴孔的吸收能力;利用扫描电镜、透射电镜和免疫胶体金技术,观察雌、雄激素作用下小鼠卵巢囊淋巴孔的变化。结果雌激素能使小鼠卵巢囊淋巴孔开放增加,淋巴孔吸收能力增强;雄激素则使淋巴孔开放数量减小,降低了淋巴孔吸收能力。实验首次发现,卵巢囊内层间皮细胞核内存在雌激素受体。雌激素干预使间皮细胞雌激素受体表达水平下降,而雄激素则上调雌激素受体的水平。结论性激素能调控小鼠卵巢囊淋巴孔的开放及淋巴吸收,其作用机制可能与卵巢囊内层间皮细胞的雌激素受体表达水平有关。Objective To investigate the effect of steroid hormones on lymphatic stomata of ovarian bursa and fluid adsorption. Methods The lymphatic adsorption was traced by trypan blue, and the ultrastructure of the lymphatic stomata and estrogen receptor level of mesothelial cells on ovarian bursa was observed by scanning electron microscopy, transmission electron microscopy and immuno-colloid golden technology. Results The absorption and opening area of lymphatic stomata were enhanced in estrogen group mice, and decreased in androgen group as compared to control group mice. Estrogen receptor was first identified in the nuclei of mesothelial cells on the inner layer of mice ovarian bursa and the level was down-regulated by estrogen and slightly up-regulated by androgen. Conclusion The absorption and the opening area of lymphatic stomata can be regulated by steroid hormones in mice, and the machanism may be related to the expression levels of estrogen receptor in the nuclei of mesothelial cells.

关 键 词：淋巴孔 卵巢囊 激素 雌激素受体 电镜 免疫胶体金技术 小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]