中药“益肾清火”方对骨髓基质细胞生物学活性的影响  

Effect of Yishenqinghuo Compound on Biological Characteristics of Rat Bone Marrow Stromal Cells

作　　者：罗礼君[1] 束蓉[1] 俞瑾[2] 乔伟伟[3] 周晓健[1] 

机构地区：[1]上海交通大学附属第九人民医院口腔内科,上海200011 [2]复旦大学附属妇产科医院中西医结合科,上海200011 [3]复旦大学实验动物科学部,上海200032

出　　处：《华西口腔医学杂志》2006年第4期346-349,共4页West China Journal of Stomatology

基　　金：上海市自然科学基金资助项目(03ZR14081)

摘　　要：目的采用血清药理学方法,观察中药“益肾清火”方对骨髓基质细胞(BMSCs)生物学活性的影响,探索该方在牙周炎骨修复中的作用和机理。方法选择12—15月龄SD大鼠,分为A、B、C、D、E共5组。A组为空白对照组,B组为牙周炎造模组,C组为造模后灌服中药高剂量组,D组为造模后灌服中药中剂量组、E组为造模后灌服中药低剂量组。牙周炎造模后灌胃10d,采集大鼠血清作用于体外培养的BMSCs,观察BMSCs增殖活性和碱性磷酸酶产生情况的变化。结果C组最后一次灌胃后0.5h采集的血清与A组血清作用相近,对细胞增殖作用最强,与其余各组有统计学差异(P<0.01),而B组作用最差;C组最后一次灌胃后1h采集的血清对细胞碱性磷酸酶的产生及活性影响较其余各组更强(P<0.01)。结论中药“益肾清火”方对牙周炎动物BMSCs有促增殖和诱导成骨分化的作用。Objective To evaluate the effect of Yishenqinghuo recipe on biological characteristic of rat bone marrow stromal ceils （BMSCs） and search for the function and mechanism of this recipe in treatment of periodontitis. Methods 12- to 15-month-old SD rats were allocated into 5 groups. Group A： control group（with no periodontitis model, fed with the same dosage of saline as Group D）; Group B： model group（with periodontitis model, fed with the same dosage of sahne as Group D）; Group C： high dosage group （with periodontitis model, fed with a high dosage of medicine）; Group D： middle dosage group（with periodontitis model, fed with a middle dosage of medicine）; and Group E： low dosage group （with periodontitis model, fed with a low dosage of medicine）. Ten days later, serums were collected from all the five groups for in vitro cultivation of BMSCs. Results There was displayed a similarity in effect between the serum collected from Group C1（serum collected 0.5 h after the last garage of Group C） and that from Group A after the last garage, the effect being the best in terms of proliferation of BMSCs. A comparison with the other groups had revealed a striking difference （P〈0.01）, with Group B having turned out to be the worst. The serum from Group C2（serum collected 1 h after the last gavage of Group C） after the last gavage could best enhance the generation and activity of ALP, having demonstrated a significant difference （P〈0.01） in comparison with the other groups. Conclusion Yishenqinghuo recipe can improve the proliferation of BMSCs and facilitate the differentiation to osteoblast.

关 键 词：牙周炎 中医药 血清药理学 骨髓基质细胞 碱性磷酸酶 

分 类 号：R285.5[医药卫生—中药学]