小脑颗粒神经元低钾性凋亡过程中Arnt2核浆转位的分析  

Arnt2 nucleocytoplasmic translocation in the process of CGNs apoptosis induced by low potassium

作　　者：孙林光[1] 银巍[1] 程文芳[2] 黄奕俊 苏兴文[1] 邱鹏新[1] 颜光美[1] 

机构地区：[1]中山大学基础医学院药理教研室 [2]中山大学第一附属医院肾内科实验室,广东广州510089

出　　处：《中国病理生理杂志》2006年第8期1610-1613,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30472010);广东省自然科学基金团队项目(No.039191)

摘　　要：目的:分析大鼠小脑颗粒神经元(CGNs)“低钾”性凋亡过程中Arnt2亚细胞定位的变化。方法:W estern b lotting法分析“低钾”性凋亡过程中CGNs核抽提物中Arnt2蛋白质的表达变化,间接免疫荧光结合激光扫描共聚焦显微术(LSM)分析Arnt2蛋白质亚细胞定位的变化。结果:在CGNs细胞核抽提物中,“低钾”诱导30 m in后Arnt2蛋白质已明显表达上调,1 h后达至峰值水平,“低钾”诱导6 h后上调表达的Arnt2回落至正常对照水平;LSM分析显示位于正常CGNs细胞核内的Arnt2在“低钾”作用下逐步向胞浆转移,并在CGNs凋亡末期与胞浆线粒体定位的HSP60完全重叠。结论:Arnt2在小脑颗粒神经元“低钾”性凋亡过程中发生了核浆转位;结果提示,Arnt2很可能是通过传递“低钾”诱发的细胞凋亡或存活信号而直接参与小脑颗粒神经元“低钾”性凋亡的调控。AIM. To analyze the changes of Arnt2 subcellular localization in the process of cerebellar granule neurons （CGNs） apoptosis induced by low concentration of potassium. METHODS： Western blotting was used to detect the variation of Arnt2 proteins in nuclei extracts of CGNs in the process of apoptosis induced by low concentration of potassium. Laser scanning confocal microscopy （LSM） was used to detect the changes of Arnt2 subcellular localization. RESULTS ： In nuclei extracts of CGNs, when treated with low concentration of potassium, Arnt2 protein was up - regulated obviously at 30 min and peaked at 1 h, returned to the level of normal control at 6 h. LSM analysis result showed that Arnt2, which located in normal CGNs nuclei, was translocated into cytoplasm after induced by low concentration of potassium gradually. At the late stage of apoptosis, Arnt2 proteins located in cytoplasm and overlapped with HsP60 completely, which was regarded as a protein of mitochondria localization. CONCLUSIONS： Arnt2 protein translocates from nuclei into cytoplasm （probably into mitochondria） during CGNs apoptosis evoked by low concentration of potassium; suggesting that Arnt2 is involved in apoptosis probably by transmitting apoptotic or survival signals evoked by potassium directly.

关 键 词：神经元 基因 Arnt2 细胞凋亡 

分 类 号：Q421[生物学—神经生物学] Q291[生物学—生理学]