双色间期荧光原位杂交技术在异性间造血干细胞移植中的应用  

Application of dual-color interphase fluorescence in situ hybridization in sex-mismatched hematopoietic stem cell transplantation

作　　者：张闰[1] 李建勇[1] 潘金兰[2] 肖冰[1] 钱思轩[1] 仇海荣[1] 吴亚芳[2] 洪鸣[1] 吴汉新[1] 薛永权[2] 

机构地区：[1]南京医科大学第一附属医院血液科江苏省人民医院,210029 [2]苏州大学附属第一医院江苏省血液研究所

出　　处：《中华器官移植杂志》2006年第8期473-475,共3页Chinese Journal of Organ Transplantation

基　　金：江苏省基础研究重大招标项目(160DC0401);江苏省社会发展项目(160DB0301);江苏省卫生厅医学科技发展项目(H200313)

摘　　要：目的探讨双色间期荧光原位杂交(FISH)法在检测异性异基因造血干细胞移植后植活标志及微量残留病灶(MRD)中的作用。方法采用X、Y染色体探针和间期双色间期FISH法检测35例异性异基因造血干细胞移植受者移植后不同时期的性染色体荧光杂交信号。并设对照组,分析检测的有效率。结果35例受者移植后的细胞遗传学及双色间期FISH性染色体分析均可见植入证据。当受者出现原病复发时,双色间期FISH性染色体可检测出受者源克隆的增加;其再次缓解后受者源克隆也相应减少。在移植后不同时间,当染色体分析为100%XX或100%XY结果时,双色间期FISH可显示不同比例的受者源染色体。结论双色间期FISH应用于异性异基因造血干细胞移植后植活标志及MRD检测,操作简单快速,是细胞形态学及细胞遗传学检查很好的补充。Objective To evaluate the significance of dual-color interphase fluorescence in situ hybridization （FISH） in the engraftment estimation and minimal residual disease （MRD） monitoring after sex-mismatched allogeneic hematopoietic stem cell transplantation （allo-HSCT）. Method The fluorescence signal of samples from 35 cases in different periods after sex-mismatched allo-HSCT were detected by interphase FISH using chromosome enumeration probes （CEP） X and Y. Results All of 35 patients had been determined to obtain engraftment after allo-HSCT. When the disease relapsed, FISH showed that the percentage of donor chromosomes was decreased and when the disease got remission, the percentage of donor chromosomes increased. When conventional cytogenetics showed 100 % XX or 100% XY, FISH showed different percentage of host chromosomes. Conclusions The test of dual-color interphase FISH is reliably sensitive and simple for engraftment evaluation and MRD monitoring post HSCT. It is a good complement method to cell morphology and traditional karyotype analysis.

关 键 词：原位杂交 荧光 造血干细胞移植 肿瘤 残余 

分 类 号：R55[医药卫生—血液循环系统疾病]