β_2糖蛋白Ⅰ与乙型肝炎病毒嗜肝性的关系  

Studies on the association between beta 2-glycoproteinⅠand hepatotropism of hepatitis B virus

作　　者：高沿航[1] 高普均[1] 王丹[1] 时阳[1] 唐彤宇[1] 朴云峰[1] 杨翰仪[2] 

机构地区：[1]吉林大学第一医院消化科,长春130021 [2]吉林大学基础医学院生物化学教研室

出　　处：《中华肝脏病杂志》2006年第8期569-571,共3页Chinese Journal of Hepatology

基　　金：国家自然科学基金(30572106)

摘　　要：目的研究乙型肝炎表面抗原(HBsAg)与β_2糖蛋白Ⅰ(β_2GPⅠ)的结合特性,探讨β_2GPⅠ在乙型肝炎病毒(HBV)嗜肝性中的作用。方法采用Western blot技术,研究还原型与非还原型β_2GPⅠ与HBsAg的结合特性。结果还原型与非还原型β_2GPⅠ与HBsAg有相同的亲和力,推测β_2GPⅠ与HBsAg的结合部位是在一级结构上相邻的氨基酸残基肽段,与空间构象关系不大。结论HBV与β_2GPⅠ有特异的亲和能力,这一亲和能力可能在HBV感染肝细胞过程中发挥了重要作用,是HBV嗜肝性的原因之一。Objective To further study the binding character of hepatitis B surface antigen （HBsAg） and beta 2- glycoprotein Ⅰ （ β2GPⅠ ） and to explore whether β2GPⅠ plays an important role in the hepatotropism of hepatitis B virus. Methods Using Western blot technique, we observed the binding character of the HBsAg with reduced and non-reduced β2GPⅠ . Results rHBsAgs with reduced and non-reduced β2GPⅠ showed identical binding activity. Conclusions The binding activity of HBsAg is dependent on tandem residues, but not on conformational structures of β2GPⅠ . There is a specific binding between HBV and β2GPⅠ, which may play an important role in HBV infection and is one of the reasons of hepatotropism of HBV.

关 键 词：肝炎病毒 乙型 肝炎表面抗原 乙型 β2糖蛋白Ⅰ 

分 类 号：R373.21[医药卫生—病原生物学]