精子超活化结构蛋白——Catsper表达特性研究  

Expression Characteristics of Mouse Catsper——the Structural Protein of Sperm Hyperactivation

作　　者：杨媛媛[1] 王根林[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《中华男科学杂志》2006年第8期675-680,共6页National Journal of Andrology

基　　金：国家自然科学基金(30471243)

摘　　要：目的：Catsper是精子特有的一种阳离子通道蛋白，对于精子超活化有重要的调节作用，为进一步了解其表达特性，本文主要研究其组织特异性表达及表达规律。方法：运用RT-PCR和免疫组织化学方法分析Catsper在2-8周龄昆明小鼠睾丸、附睾等8种组织中的表达情况，在精子上的精确定位以及随发育阶段变化表达量的变化。结果：Catsper特异性地表达于睾丸和附睾组织上；主要定位于精子尾部的主段；Catsper的表达量受到发育阶段的调控，该蛋白表达量随个体发育周龄的增长而呈增加的趋势，于3周龄开始检测到它的表达，随后表达量显著增加至6周龄，以后逐渐增长至一个平台期。结论：小鼠的Catsper基因特异性地表达于睾丸和附睾上，并且主要位于精子尾部的主段，其表达量与小鼠的发育阶段有密切的关系。Objective ： To study the expression specificity in tissues of the Catsper and its expression rules, which is a specific cation channels in sperm and plays an important role in sperm hyperactivation. Methods： The expression of Catsper in eight different tissues was examined by RT-PCR and immunohischemistry, the location of Catsper in sperm determined by immunofluorescence and the expression changes of Catsper analyzed in mice of different age groups. Results ： Catsper was expressed specifically in the testis and epididymis, and was mainly located in the principal piece of the sperm tail. Catsper gene expression was developmentally regulated with a direct correlation between Catsper expression and mouse sexual maturation. Catsper gene experssion was first detected at 3 weeks of age and increased significantly till 6 weeks of age and then gradually to a stationary phase. Conclusion ： Catsper expresses in the testis and epididymis, and its growth period and expression amount are closely related with the developmental stages of the mouse.

关 键 词：精子超活化 Catsper RT-PCR 睾丸 小鼠 

分 类 号：Q492[生物学—生理学]