草鱼催乳素抗血清的制备与鉴定  

PREPARATION AND IDENTIFICATION OF ANTIBODY AGAINST GRASS CARP PROLACTIN

作　　者：陈松林[1] 陈细华[1] 邓文涛[1] 夏盛芹[1] 

机构地区：[1]中国水产科学研究院长江水产研究所淡水鱼类种质资源与生物技术国家重点实验室

出　　处：《动物学杂志》1996年第5期35-37,共3页Chinese Journal of Zoology

摘　　要：应用从草鱼垂体中分离纯化的催乳素（ＰＲＬ）免疫兔子制备了特异抗血清。用常规酶联免疫吸附测定法（ＥＬＩＳＡ）检测表明该抗血清与大马哈鱼生长激素（ｓＧＨ）及促性腺激素（ｓＧｔＨ）完全没有交叉反应，而与草鱼生长激素（ｇｃＧＨ）有微弱交叉反应。免疫细胞化学染色表明该抗血清主要与草鱼垂体前叶（ＲＰＤ）催乳素细胞发生结合反应，与垂体问叶（ＰＰＤ）细胞有微弱染色反应，但与神经垂体（ＮＰ）及垂体后叶（ＰＩ）细胞则完全没有免疫结合反应。The antibody against grass carp(Ctenopharyngodon idellus) prolactin(gcPRL)was prepared by 4 injections of gcPRL into rabbit.The titre of the antiserum was determined to be 1:50000 by enzylnelinked immunosorbent assay(ELISA). ELISA showed that the antiserum mainly reacted with gcPRL,not cross reacted with salmon growth hormone and gonadotropin.Immunocytochemistry demonstrated that the antiserum mainly stained the cells in rostral pars distalis(RPD) and had a faint cross reaction with cells in proximal pars distallis (PPD),but did not stained the cells in pars intermedia(PI) and neurohypophysis(NP).

关 键 词：草鱼 催乳素 抗体 鉴定 

分 类 号：Q959.468[生物学—动物学] S965.112[农业科学—水产养殖]