延迟预适应对兔心脏缺血再灌注损伤保护作用的研究  

Protective Effects of Delayed Ischemic Preconditioning on Heart Ischemia/Reperfusion Injury in Rabbit

作　　者：孙贤林[1] 张静[2] 张新金[2] 

机构地区：[1]安徽省立医院心内科,邮政编码合肥230001 [2]昆明医学院第一附属医院心内科

出　　处：《微循环学杂志》2006年第3期28-30,F0003,共4页Chinese Journal of Microcirculation

摘　　要：目的:观察延迟缺血预适应(IschemicPreconditioning,IPC)对兔在体心脏缺血再灌注(Ischemia/Reperfusion,I/R)损伤的保护作用。方法:方法18只兔随机分为3组:假手术组(CON组,n=6),缺血再灌注组(I/R组,n=6),预适应组(IPC组,n=6)。采用免疫组化方法检测诱导型一氧化氮合成酶(iNOS)及内皮型一氧化氮合成酶(eNOS)在心肌及冠状动脉内皮中的表达情况。结果:IPC组的梗死面积明显小于I/R组。在心肌中,IPC组iNOS表达明显高于I/R组与CON组,而eNOS无显著性差异。在内皮中,IPC组eNOS表达明显高于I/R组,与CON组无显著性差异,而iNOS均无表达。结论:延迟缺血预适应能缩小兔缺血再灌注后心肌梗死面积。Objective: To study the protective effects of delayed ischemic preconditioning on heart in rabbits during ischemia/reperfusion period. Method: Eighteen rabbits were divided randomly into three groups: Control group (CON group, n=6) , ischemia/reperfusion group(I/R group, n=6) , and ischemic preconditioning group (IPC group, n=6). Through immunohistochemistry method, the expressions of endothelial NO Synthase(eNOS) and inducible NO Synthase(iNOS) in myocardium and endothelial cells were studied. Results: Infarct size of IPC group was markedly less than that of I/R group. In myocardium,there were no significant differences in all of the three groups of the expression of endothelial NO Synthase (eNOS) (P>0.05), but for the inducible NO Synthase(iNOS), IPC group was higher than that of I/R group and CON group . As to the expression of eNOS, IPC group was higher than that of IR group in endothelinal cells. The expression of iNOS was not found in all of the three groups.Conclusion: Delayed IPC reduced the Rabbit’s myocardial infarct size after Ischemia/Reperfusion injury.

关 键 词：心脏缺血再灌注损伤 心脏保护作用 延迟预适应 一氧化氮合成酶 缺血预适应 心肌梗死面积 早期预适应 生理现象 心肌细胞 冠脉内皮 

分 类 号：R654.2[医药卫生—外科学] R972[医药卫生—临床医学]