被动吸烟对子代小鼠学习记忆的影响及抗氧化物干预研究  

INFLUENCE OF PASSIVE SMOKING ON LEARNING AND MEMORY ABILITY OF MOUSE OFFSPRING AND INTERVENTION BY ANTIOXIDANTS

作　　者：杨杰[1,2] 姜丽娜[1] 袁振丽[1] 郑宇飞[1] 李君文[3] 

机构地区：[1]吉林大学公共卫生学院 [2]卫生学环境医学研究所,天津300050 [3]卫生学环境医学研究所

出　　处：《营养学报》2006年第4期314-317,共4页Acta Nutrimenta Sinica

摘　　要：目的:研究烟雾环境对孕鼠的子代智力发育的影响及抗氧化剂的神经系统保护作用。方法:雌性小鼠20只,随机均分为吸烟(TS),吸烟+槲皮素(TS+Q,quercetin80mg/kgdiet),吸烟+VE(TS+VE,100mg/kgdiet)和对照4组,前3组暴露于香烟的雾染毒柜中6h/d,5d/w,连续3个月,之后每组放1只雄鼠进行交配,雌鼠继续染毒至孕20d左右待产时,产仔后,选子代雄鼠每组15只,分别给予相应饲料至仔鼠体重20±2g时进行水迷宫试验,测定其仔鼠空间学习记忆能力、长时程增强(LTP)和脑组织中一氧化氮(NO)含量和一氧化氮合酶(NOS)、乙酰胆碱脂酶(AChE)活性、血清维生素E(VE)浓度和活性氧(ROS)的影响及VE的干预效果。结果:仔鼠水迷宫测试6d后,对照组和TS+VE、TS+Q干预组小鼠到达终点平台所需时间(潜伏期)和进入盲端的次数(错误次数)明显低于TS组(P<0.05)。10d后,TS组与对照组和TS+VE干预组潜伏期比差异显著(P<0.05)。TS组LTP增强受到抑制,与对照组比差异显著(P<0.05)。TS组子代小鼠脑组织NOS活性明显高于对照组(P<0.05),TS+VE干预组和TS组与其它组比,NO的含量差异显著(P<0.05)。血清VE浓度和ROS活性与子代小鼠学习记忆能力的测定结果相一致。结论:孕期被动吸烟可降低仔鼠大脑海马齿状回(DG)神经突触的可塑性及神经元的兴奋性,抑制LTP的形成,使学习记忆功能下降,VE干预可得到改善。Objective ： To study the protective effects of two antioxidants on the influence of passive smoking on learning and memory ability of mouse offspring. Method： Passive smoking model of pregnant mice was established. Learning and memory ability was evaluated by water maze and long term potentiation （LTP）. Nitric oxide （NO） content and nitric oxide synthase （NOS） activity of brain, vitamin E（VE） concentration and reactive oxygen species （ROS） of serum were determined. Results： Latency （the swimming time from beginning to endpoint） and errors （the number of entering blind end） in control and two antioxidant groups were shorter as compared with tobacco smoking （TS） group after 6 d in water maze test, and still shorter after 10 d in control and TS＋VE groups. LTP was inhibited in TS group but increased significantly in two antioxidant groups. NOS activitiy was significantly higher in TS group in comparison with the control. NO content of TS＋VE group was significantly lower than TS and TS＋Q groups. Serum VE concentration and ROS level were correlated with the results of latency in water maze and LTP. Conclusion： Passive smoking of the pregnant mice may restrain LTP formation through disturbance of hippocampus function, and reduce the learning and memory ability of the offspring and VE may protect such effects.

关 键 词：被动吸烟 学习记忆能力 VE 槲皮素 LTP 

分 类 号：R114[医药卫生—卫生毒理学]