以减毒沙门氏菌为载体的GM-CSF与生长抑素融合表达质粒对小鼠淋巴细胞增殖和GH及IGF-Ⅰ分泌的影响  

Lymphocyte Proliferation and GH,IGF-ⅠSecretion of Mice Immunized with Fusion-Expression Plasmid of GM-CSF and Somatostatin Delivered by Attenuated Salmonella

作　　者：舒邓群[1] 茆达干[1] 吴志敏[2] 程宝[1] 杨利国[1] 

机构地区：[1]南京农业大学动物繁育研究所,南京210095 [2]江西农业大学动物科技学院,南昌330045

出　　处：《畜牧兽医学报》2006年第8期814-818,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30270959);南京农业大学校级青年创新基金(y200411)

摘　　要：40只22日龄小鼠，雌雄各半，平均分为4组。其中第1组为对照组，第2～4组分别经口给予以减毒沙门氏菌为载体的pcS／2SS、pGM—CSF／SS和pGM—CSF＋pcS／2SS DNA疫苗，剂量10^9CFU／只，2周后以相同剂量加强免疫一次，分析不同DNA疫苗对小鼠淋巴细胞增殖和GH、IGF-Ⅰ分泌的影响。结果表明GM—CSF可增强免疫鼠脾淋巴细胞对特异性抗原刺激的增殖能力，以pGM—CSF／SS免疫组最强；DNA疫苗免疫组的GH和IGF-Ⅰ分泌水平都高于对照组。这些结果证明，GM—CSF对生长抑素DNA疫苗有一定的免疫增强作用，以减毒沙门氏菌为载体介导DNA疫苗通过口服途径免疫动物是一种较好的免疫方式。pGM—CSF／SS的免疫效果优于pGM—CSF＋pcS／2SS。To study the immuno-enhancement of genetic adjuvant GM-CSF on somatostatin （SS） DNA vaccine, a total of 40 mice at the age of 22 days were divided into 4 groups and immunized with salt solution （group 1）, pcS/2SS （group 2）, pGM-CSF/SS （group 3） and pGM-CSF＋pcS/2SS（group 4） delivered by attenuated Salmonella at a dose of 10^9CFU per mouse, respectively. A booster was given 2 weeks later with same dosage and method. The results are as follows： Lymphocyte proliferation response of group 3 was higher than the others, indicating that GM-CSF can enhance lymphocyte proliferation responses; the GH level in plasma of group 3 and IGF-Ⅰ level in plasma of group 4 are higher than that of other groups, and levels of GH and IGF-Ⅰ begin to rise at the 2nd and 4th week post primary immunization, respectively. We conclude that genetic adjuvant GM-CSF can enhance immuno-function of somatostatin DNA vaccine. Immune response of fusion-expression plasmid of GM-CSF and SS is superior to that of co-immunization of pGM-CSF＋pcS/2SS.

关 键 词：GM—CSF 生长抑素 减毒沙门氏菌 淋巴细胞增殖 GH IGF-Ⅰ 

分 类 号：S852.2[农业科学—基础兽医学] R392.1[农业科学—兽医学]