STZ诱导小鼠糖尿病模型中T、B淋巴细胞功能的变化  

The alteration of T,B cell response in IDDM mice induced by multiple streptozotoxin

作　　者：杨巍[1] 刘丹[1] 于春雷[1] 王莉[1] 李一[1] 

机构地区：[1]吉林大学基础医学院免疫学教研室,长春130021

出　　处：《中国免疫学杂志》2006年第8期754-757,共4页Chinese Journal of Immunology

摘　　要：目的:探讨多次小剂量注射链脲佐菌素(STZ)诱导的糖尿病模型小鼠中T、B淋巴细胞功能的变化及可能的机制,为小鼠糖尿病模型的应用提供实验依据。方法:①采用腹腔注射链脲佐菌素(STZ)诱导昆明小鼠糖尿病作为动物模型。②用ConA和Insulin分别刺激脾和胸腺T细胞,采用MTT法检测成熟与未成熟T细胞对于丝裂原和特异性抗原的增殖转化能力。③采用ELISA间接法测定血清中Insulin自身抗体(IAA)的水平。④采用ELISA夹心法测定小鼠脾细胞及胸腺细胞培养上清的IL4与IFNγ的含量。结果:①糖尿病模型组血清中Insulin自身抗体(IAA)含量明显高于正常组(P<0.05)。②模型组成熟T细胞(脾脏细胞)对于ConA刺激的增殖能力较弱(P<0.05),对于Insulin刺激的增殖能力与正常组没有显著差别(P>0.05);模型组未成熟T细胞(胸腺细胞)对于ConA刺激的增殖能力与正常组没有显著差别(P>0.05),而对于Insulin刺激的增殖能力较高(P<0.05)。③模型组脾细胞培养上清的IFNγ的含量明显高于对照组(P<0.05),而胸腺细胞培养上清中IFNγ的含量在模型组与对照组之间无明显差异,IL4在实验组和对照组均未检出。结论:多次小剂量注射STZ诱导糖尿病小鼠模型是自身免疫应答所导致;自身免疫性T、B细胞的功能及自身抗体均有增强;Th1介导的细胞免疫在STZ诱导的糖尿病中发挥着重要作用。Objective ：To study the alterations of T and B lymphocytes and its possible mechanisms in IDDM induced by multiple low-dose injection of streptozotoxin. Methods： （ 1 ） KM mice diabetes mellitus were triggered by five daily peritoneal injection of streptozotocin. （2）T cells from spleen and thymus were activated by ConA and Insulin respectively and detected their proliferation ability to the stimulators by MTT method. （3）Insulin autoantibody （1AA） in serum were detected with indirect enzyme-linked immunosorbent assay（ELISA）. （4）The concentrations of IL-4 and IFN-γ in mitogen-stimulated thymocytes and spleenocytes supematants were measured by Sandwich ELISA. Results：（ 1 ）The level of autoantibody in diabetes group was higher than control group（P 〈 0. 05 ）. （2） Mature T cells（spleenoeytes） proliferation defect in STZ-induced diabetes mellitus mice compared with controls in response to ConA （P 〈 0. 05 ）, but no difference to insulin （P 〉 0. 05 ）. The immature T cells （thymoeytes） are reversed. （3） In spleenocytes supematants,the quantity of IFN-γ in diabetes groups highly increased than control groups, but in thymocytes supematants,there were no difference of IFN-γ between diabetes group and control group. We didn＇t detect the IL-4 both in model and control group with this ELISA kit. Conclusion： It is a result of self immune response in which multi-low dose injection STZ induced diabetes mellitus of mice. The functions of autoreactive T and B cells were enhanced, and the autoantibodies also showed increase. Cell immune response mediated by Thl cells plays an important role in diabetes mellitus induced by STZ.

关 键 词：STZ IDDM 自身反应性T细胞 自身抗体 IL-4 IFN-γ 

分 类 号：R392[医药卫生—免疫学]