甲基强的松龙对免疫复合物型肾炎大鼠细胞外基质的影响  

The influence of methyl-prednisone on extracellular matrix in rats with immune commplexes nephritis

作　　者：孙广东[1] 刘树军[1] 苗里宁[1] 顾华[1] 罗萍[1] 

机构地区：[1]吉林大学第二医院肾内科,长春130041

出　　处：《中国免疫学杂志》2006年第8期758-760,共3页Chinese Journal of Immunology

基　　金：吉林省科委资助项目(990570)

摘　　要：目的:观察甲基强的松龙治疗免疫复合物型肾炎大鼠后肾小球细胞外基质成份的变化及其与PAI1含量改变的关系及该药的效果。方法:以阳离子化小牛血清白蛋白(CBSA)诱导的肾炎大鼠模型,以ELISA方法检测不同疗程组的大鼠肾组织中的FN、LN的含量,发色底物法检测肾组织中PAI1含量变化,并以24小时尿蛋白定量评价甲基强的松龙的治疗作用。结果:模型组大鼠肾组织的FN、LN及PAI1含量较正常组显著升高,且FN、LN与PAI1的含量呈正相关关系,肾小球系膜基质轻、中度增生;经甲基强的松龙治疗1周及2周后,治疗组的FN、LN及PAI1含量较模型组明显下降,肾小球系膜基质增生不同程度减轻,但未达到正常组水平;治疗后大鼠尿蛋白含量显著降低。结论:免疫复合物肾炎大鼠细胞外基质积聚与PAI1含量升高密切相关,甲基强的松龙可能通过干扰PA/PAI1系统而影响细胞外基质积聚并达到治疗作用。Objective：To observe the theraputic effect and changes of glomerular extracellular matrix components （ECM） and PAI-1 and their relationships after cure with methyl-prednisone on immune complexes nephritis rats. Methods：Immune complexes nephritis rats model were induced with C-BSA. Levels of FN ,LN in different treatment groups were analyzed by ELISA, level of PAI-1 in rats renal tissue was determined by color developing substrate ,was theraputic effect of methyl-prednisone with 24 hours volumes of urine protein. Results：The levels of PAI-1 ,FN and LN of model groups were significantly higher than those of normal and control groups,and PAI-1 was significantly correlated with LN and FN, Glomerular mesanglal matrix proliferated slightly and moderately ;The levels of FN, LN and PAI-1 decreased signifcantly and glomerular mesangial matrix proliferation lessen differently after cure of methyl-prednisone for 1 and 2 weeks, but not reaching the level of normal groups ;24 hours volumes of urine protein decrease significantly in treatment groups. Conclusion ：The ECM accumulation correlate with PAI-1 increase in immune complexes rats, methyl-prednisone may affect ECM accumulation by interfering with PA/PAI-1 system to reach a treatment purpose.

关 键 词：甲基强的松龙 免疫复合物型肾炎 细胞外基质 纤溶酶原激活物抑制剂-1 

分 类 号：R692.3[医药卫生—泌尿科学]