胶体染料试纸条法检测牛血吸虫病的进一步研究  

Further study on detection of cattle schistosomiasis with dipstick dye immunoassay

作　　者：何伟[1] 朱荫昌[1] 曹国群[1] 

机构地区：[1]江苏省血吸虫病防治研究所,无锡214064

出　　处：《中国血吸虫病防治杂志》2006年第4期270-272,共3页Chinese Journal of Schistosomiasis Control

基　　金：江苏省卫生厅项目(X2109)

摘　　要：目的评价胶体染料试纸条法(DDIA)检测牛血吸虫病的效果。方法采用5、10、20μl不同血清检测量进行DDIA检测,以确定DDIA检测牛血吸虫病的最适条件。以确定的最适条件对血吸虫病牛、阴性对照牛和肝片形吸虫病牛的血清进行检测并和环卵沉淀试验(COPT)检测结果进行比较。结果检测血清量为10μl时,DDIA的敏感性、特异性和与肝片形吸虫的交叉反应率分别为95.7%、96.0%和20.0%;COPT的敏感性、特异性和与肝片形吸虫的交叉反应率分别为72.9%、100.0%和0。结论当检测血清量为10μl时,DDIA检测牛血吸虫病有较高的敏感性和特异性,且敏感性高于COPT。Objective To evaluate the effect of DDIA on detecting cattle schistosomiasis. Methods The sera of schistosomiasis cattle, negative control cattle and fascioliasis cattle were detected with DDIA and compared with COPT. Results The sensitivity, specificity and across reaction of DDIA using 10 μl of sample sera were 95.7%, 96.0% and 20. 0% respectively and COPT were 72.9%, 100. 0% and 0 respectively. Conclusions DDIA has high sensitivity and specificity for detecting cattle schistosomiasis with 10 μl of sample serum, and the sensitivity is higher than that of COPT.

关 键 词：胶体染料试纸条法 环卵沉淀试验 牛血吸虫病 免疫诊断 

分 类 号：R532.21[医药卫生—内科学]