反相高效液相色谱法测定感冒康胶囊中脱水穿心莲内酯含量  

Determination of Dehydroandrographolide in Ganmaokang Capsule by RP-HPLC

作　　者：李彬 郑国平 

机构地区：[1]浙江省舟山市药品检验所,浙江定海316000

出　　处：《中国药业》2006年第13期39-40,共2页China Pharmaceuticals

摘　　要：目的用反相高效液相色谱法(RP-HPLC法)测定感冒康胶囊中脱水穿心莲内酯含量。方法采用Hypersil ODS C_(18)柱(250mm×4．6mm,5μm)为色谱柱,以甲醇-水(60∶40)为流动相,流速1．0mL/min,检测波长254nm。结果脱水穿心莲内酯浓度在0．0101～0．3232mg/mL范围内与峰面积线性关系良好(r=1．0000),平均加样回收率为98．2%(RSD=0．9%),重现性试验RSD为1．2%。结论RP-HPLC法操作简便、测定结果准确、重现性好,可作为感冒康胶囊的含量测定方法。Objeetive To develop the RP- HPLC method for determination of dehydroandrographolide in Ganmaokang capsule. Methods The column was ODS Hypersil C18（250 mm ×4.6 mm, 5 μm）;mobile phase was methanol-water （60：40）. The wavelength of detection was 254 nm. Results Linearity range was 0.0101-0.3232mg/mL （r=1.0000）.The average recovery was 98. 2% （ RSD=0. 9% ）. RSD of the precision was 1.2%. Conclusion The method is simple, accurate, reproducible and can be used for content determination of Ganmaokang capsule.

关 键 词：感冒康胶囊 脱水穿心莲内酯 反相高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]