组织凝集素在人染色体上的基因定位  

Localization of Sarcolectin Gene on Human Chromosome

作　　者：吴秋茜[1] 蒋盘宏[1] 

机构地区：[1]北京化学试剂研究所生化试剂高技术实验室,北京100022

出　　处：《遗传》1996年第5期36-38,共3页Hereditas（Beijing)

摘　　要：本文用非放射性DIG标记组织凝集素基因操针研究其在人染色体上的基因定位，首先对基因原位杂交条件进行了一系列研究．结果表明采用染色体温和变性、基因片段探针等为较好的基因原位杂交条件，通过135个细胞中期相的分析结果显示，杂交阳性率为20％，本底为2．81，杂交点分布峰位于3q12－13．The localization of sarcolectin gene on human chromosome is studied. A modifled system of in situ hybridization was developed. A specific signal was generated by cmploying a nonradioactive DIG-labelled gene fragment as a probe in combination with the hybridisation buffer containing no dextran sulphate and softly denatured chromosomes. Using this system, unique sequcnce for sarcolectin assmall as 0.65kb was detectable and observed at 3q 12-13. Of 135 metaphases examined, 27(20%) had at least one grain deposited on 3q 12-13, and the backgrand was 2.81.

关 键 词：组织凝集素 人染色体 基因定位 

分 类 号：Q513.2[生物学—生物化学] Q343.2