乙肝病毒增强子对其基因表达的调控  

Regulation of Hepatitis B Virus Gene Expression by Its Two Enhancers

作　　者：傅磊[1] 吴雪[1] 孔玉英[1] 汪垣[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《生物化学与生物物理学报》1996年第6期590-599,共10页

基　　金：中国科学院重点基金

摘　　要：本文构建了线性化的含有从核心抗原启动子Ｃｐ起始的ＨＢＶ完整转录单元的基因组克隆，以此为模型，通过增强子工（ＢＮＩ）和增强子ＩＩ（ＥＮＩＩ）的缺失或点突变分析，研究ＥＮＩ和ＥＮＩＩ对ＨＢＶ基因组基因表达的调控．结果表明ＥＮＩ和ＥＮＩＩ对Ｓ基因表达均有增强作用，且相互协同。两个增强子对ｅ抗原的表达也有增强作用，一但ＥＮＩ２Ｃ和Ｅｐ的缺失对ｃ抗原的表达几乎没有影响。ｃ抗原的表达主要由ＥＮＩＩ调控，ＥＮＩ与ＥＮＩＩ有协同作用．ＥＮＩ和ＥＮＩＩ对编码两个功能不同的３．５ｋｂｍＲＮＡ的转录调控可能是相互协同且存在分工的。A linearized genome containing the entire HBV 3.5kb mRNA transcriptional units was constructed. Based on it,a seriea of mutants with enhancer delet ions or point mutations were generated.This system has served as a good model to study the regulation of hepatitis B virus gene expression by its two enhancers(ENI andENII).The results showed that both ENI and ENII could inerease the expression of HAsAg, and the two enhancers worked synergistically.They also couldincrease the expression of HBeAg, but the deletion of 2C and Ep of ENI had no effect on the expression of HBe/cAg in the cytoplasm. The expression of C gene might be regulated mainly by ENII,but ENI could cooperate with the action of ENII.The data indicatedthat ENI and ENII might function differentially but cooperatively in regulating the transcription of the two 3.5kb mRNAs which functioneddistinctively.

关 键 词：乙型肝炎病毒 增强子 基因表达 调控 

分 类 号：R373.21[医药卫生—病原生物学]