人红细胞膜上阴离子交换蛋白和葡萄糖运输蛋白的相互影响  

Interaction Between the Anion Exchange Protein and the Glucose Transport Protein in Human Red Cell Membranes

作　　者：张志鸿[1] 彭锋[1] 李晓泉 周汉清[1] 胡小健[1] 

机构地区：[1]复旦大学生理学和生物物理学系

出　　处：《生物化学与生物物理学报》1996年第6期665-670,共6页

基　　金：国家自然科学基金

摘　　要：用细胞松弛素Ｂ抑制红细胞膜上葡萄糖运输蛋白（ＧｌｕＴ－１）对葡萄糖的运输，观察到对阴离子运输有促进作用。当ＧｌｕＴ－１结合其底物分子－葡萄糖后同样加快了阴离子运输速率．另一方面，实验办给出了葡萄糖跨膜运输特性和Ｃｌ－离子浓度的关系，表明随着Ｃｌ－离子浓度的加大能使葡萄糖运输过程中Ｋｍ减小、Ｖ（ｍａｘ）增大。这些结果表明了在完整红细胞膜上阴离子交换蛋白Ｂａｎｄ３和ＧｌｕＴ－１之间存在着双向联系，即一种膜蛋白的构象改变能影响另一种膜蛋白的功能。Cytochalasin B was used to inhibit the glucose iransport through the glucose transport protein(GluT-1)in red cell membranes.It was observed that the anion transport was increased at the same time.The rate of anion transport was also inereased when GluT-1 was bound with its substrate molecule-glucose.On the other hand,the experimental result on the relationship between glucose transport and Cl-concentration showed that Km was decreased and Vmax increased following the increase of Cl-concentration. There would be a bidirectional linkage between Band 3(anion exchange protein)and GluT-1 in the intact red cell membranes.In other words,the conformation change of membrane protein could affect the function of other kinds of membrane proteins.

关 键 词：生物膜 红细胞 阴离子交换蛋白 葡萄糖运输蛋白 

分 类 号：Q734[生物学—分子生物学]