光亲和标记鉴定玉米根脱落酸结合蛋白  

Identification of the Photoaffinity-labelled Abscisic Acid Binding Proteins from Maize Root Microsome

作　　者：吴忠义[1] 陈珈[1] 朱美君[1] 

机构地区：[1]北京农业大学生物学院

出　　处：《生物化学与生物物理学报》1996年第6期694-696,共3页

基　　金：国家自然科学基金

摘　　要：光亲和标记鉴定玉米根脱落酸结合蛋白吴忠义，陈珈，朱美君（北京农业大学生物学院，１０００９４）关键词结合蛋白；光亲和标记；ＡＢＡ；受体；微粒体脱落酸（ＡＢＡ）作为一大类植物激素，在高等植物的生长发育以及对逆境的适应过程中发挥着重要作用。在探讨激素作用的...ABA binding protein(ABA-BP)was solubilized with 0.2%(W/V)Triton X-100 from the micronmal membrane of maize root,with a speciffc bind ing activity of 0.56 pmol ABA/g FW.When ABA was cross-linked to its binding sites by irradiating the microsomal proteins with light of～330nm after incubation with low levela of H3-(±)ABA,it was found that this kind of photoaffinity labelling was specific to ABA-BP.On PAGE and SDS-PAGE three major Protein bands with molecular weights of 72kD.47kD and 87kD were found with high radioactivity.

关 键 词：玉米 根 脱落酸 结合蛋白 光亲和标记 

分 类 号：S513.01[农业科学—作物学]