单细胞凝胶电泳图像分析系统1.0的设计  

Design and implementation of image analysis system on single cell gel electrophoresis

作　　者：黎建明[1] 朱志良[1] 庄志雄[2] 籍涛 肖惠贞[1] 

机构地区：[1]深圳市宝安区疾病预防控制中心,广东深圳518101 [2]深圳市疾病预防控制中心,518100 [3]深圳三九胃泰制药有限公司,518100

出　　处：《中国职业医学》2006年第4期247-248,251,共3页China Occupational Medicine

基　　金：国家自然科学基金资助项目(39970636);宝安区科技立项项目(2004048)

摘　　要：目的设计单细胞凝胶图像分析系统(IM I),并确定系统的可靠性与准确性。方法分析模拟彗星图像、用户使用情况、DNA损伤模型毒物H2O2的DNA损伤作用、被引用文献情况以及与权威的KS400彗星图像分析系统进行比较。结果IM I分析得出的尾矩准确度98.7%,H2O2引起的DNA损伤存在剂量—反应关系。IM I与KS400分析结果具有高度相关性,尾长相关系数r=0.997 6,P<0.000 1,尾矩相关系数r=0.996 4,P<0.000 1。近4年来,使用IM I作为研究工具发表的文献17篇,其中论著12篇。结论通过作者以及其他科研工作者的几年来的研究,表明IM I是一种可靠的彗星图像分析工具。Objective To design an image analysis system on single cell gel electrophoresis （IMI） and to evaluate its reliability and accuracy. Methods Some model comet images were evaluated, H2O2 -induced breakages of single strand DNA in peripheral lymphocytes were detected. A comparison between IMI and KS400 designed by Zeiss was carried out, literatures related to the usuage of IMI since 2001 were collected. Results Accuracy of IMI analysis model comet image was 98.7%. There were dose-relationship between H2O2 and indexes such as tail moment, tail length, with a good correspondence between IMI and KS400. 17 literatures on IMI were found. Conclusion IMI was a good tool for analyzing single ceil gel electrophoresis.

关 键 词：单细胞凝胶电泳 图像分析 DNA损伤 

分 类 号：R114[医药卫生—卫生毒理学] R115[医药卫生—公共卫生与预防医学]