硫酸铝钾对胎鼠肢芽细胞增殖和分化的影响  

Influence of aluminum potassium sulfate on proliferation and differentiation of mouse embryo limb bud cells

作　　者：陈雅[1] 张本忠[1] 

机构地区：[1]兰州大学公共卫生学院卫生毒理学教研室,甘肃兰州730000

出　　处：《中国职业医学》2006年第4期271-272,共2页China Occupational Medicine

摘　　要：目的研究硫酸铝钾对胎鼠肢芽细胞的增殖和分化的影响。方法应用微团培养法探讨不同浓度铝（0～100μmoL／L）对体外培养的小鼠胚胎肢芽细胞增殖和分化的影响。结果铝对肢芽细胞的50％增殖抑制浓度（IP50）和50％分化抑制浓度（ID50）分别为45．97和3．60μmol／L，IP50／ID50值为12．8，并有明显的剂量-反应关系。结论硫酸铝钾对胎鼠肢芽细胞的增殖和分化可能有抑制作用。Objective To investigate the effects of KAl（ SO4 ） 2 on proliferation and differentiation of mouse embryonic limb bud cells. Methods With the technic of mieromass culture in vitro, the effects of KAI（ SO4 ） 2 （0 - 100 μmol/L） on the chondrogenesis of mouse embryo limb bud ceils was assessed. Results The concentrations of 50% inhibition dose of irradiation for cell proliferation （IP50） and the dose giving 50% of maximal inhibition （ID50） of KAl（ SO4 ）2 were ,14.94 μmol/L and 3.60 μmol/L respectively, and the ratio of IP50 and ID50 was 12. 8. There was an obvious dose-dependent response observed. Conclusion One of the teratogenie mechanisms of KAl（ SO4 ） 2 could be the inhibition of proliferation and differentiation of mouse embryo limb bud cells.

关 键 词：肢芽细胞 微团培养 分化 增殖 硫酸铝钾 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]