野生大豆rbcS基因的克隆及结构分析  

CLONING AND STRUCTURE ANALYSIS OF rbcS GENE FROM WILD SOYBEAN

作　　者：曹凯鸣[1] 袁卫明[1] 詹树萱[1] 顾其敏[1] 徐豹[2] 

机构地区：[1]复旦大学生物化学系,上海200433 [2]吉林省农业科学院大豆研究所,公主岭136100

出　　处：《Acta Botanica Sinica》1996年第9期753-756,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：About 1 kb fragment of rbcS (ribulose 1,5 bisphosphate carboxylase small subunit) gene in wild soybean (Glycine soja, Ji 50017) was amplified from total DNA by PCR assay. Sequence analysis of the fragment indicated that 1089 bp sequenced included the whole coding region for Rubisco small subunit. The rbcS gene in wild soybean encoded a precursor composed of a transit peptide of 55 amino acids and a mature protein of 123 amino acids. There were two introns found in the rbcS gene as other dicotyledonous species previously sequenced.Comparison of DNA sequences showed high degree homology of rbcS genes between wild soybean and cultivated soybean (Glycine max var. wayne). Some changes of amino acids emerged from the diverse nucleotides did not affect the function of the small subunit. These results may contribute some basic data in molecular biology to study the origin and evolution of soybean.About 1 kb fragment of rbcS (ribulose 1,5 bisphosphate carboxylase small subunit) gene in wild soybean (Glycine soja, Ji 50017) was amplified from total DNA by PCR assay. Sequence analysis of the fragment indicated that 1089 bp sequenced included the whole coding region for Rubisco small subunit. The rbcS gene in wild soybean encoded a precursor composed of a transit peptide of 55 amino acids and a mature protein of 123 amino acids. There were two introns found in the rbcS gene as other dicotyledonous species previously sequenced.Comparison of DNA sequences showed high degree homology of rbcS genes between wild soybean and cultivated soybean (Glycine max var. wayne). Some changes of amino acids emerged from the diverse nucleotides did not affect the function of the small subunit. These results may contribute some basic data in molecular biology to study the origin and evolution of soybean.

关 键 词：大豆 野生大豆 rbcS基因 克隆 结构分析 

分 类 号：S565.103.2[农业科学—作物学]