Arthrobacter globiform is酯酶基因的克隆、表达和酶活性测定  

Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase

作　　者：李新月[1] 叶蕊芳[1] 郑黎[1] 郑一涛[1] 李晴[1] 薛建萍[2] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]上海农药研究所,上海200032

出　　处：《华东理工大学学报（自然科学版）》2006年第8期930-932,979,共4页Journal of East China University of Science and Technology

摘　　要：采用聚合酶链反应(PCR)的方法扩增了A rthrobacter g lobiform is中酯酶编码基因,与载体质粒连接后在大肠杆菌BL 21(DE 3)中进行表达。以包涵体形式表达的蛋白在8 m o l/L尿素作用下溶解,经过透析复性后获得了具有活性的粗蛋白。产物活性实验表明,酯酶表现出较高的催化活性,为菊酯作为杀虫剂的应用奠定了基础。A novel esterase which stereoselectively hydrolyzes the （＋）-trans stereoisomer of ethyl chrysanthemate to produce （＋）-trans-chrysanthemic acid was discussed. The gene coding for the esterase was amplified from Arthrobacter globiformis by polymerase chain reaction （PCR）. The gene was inserted into a vector and expressed in Escherichia coli BL21 （DE3）. The protein expressed in body was dissolve in 8 mol/L urea before dialysis. The purified crude enzyme show high catalysis activity. It may suggest the possibility that （＋）-trans-chrysanthemate is identified as the insecticide.

关 键 词：酯酶基因 克隆 表达 活性测定 

分 类 号：Q78[生物学—分子生物学]