用组织培养技术筛选杨树耐盐种质  

Selection of Salt Tolerance Poplar Regenerated Plant-lets in Tissues Cuiture

作　　者：张立钦[1] 郑勇平[1] 金佩英 

机构地区：[1]浙江林学院,浙江省林业厅,浙江省余杭市林水局

出　　处：《浙江林学院学报》1996年第4期397-404,共8页Journal of Zhejiang Forestry College

基　　金：浙江省自然科学基金

摘　　要：①在质量浓度为５．０ｇ·Ｌ￣（－１）的氯化钠胁迫条件下，杨树外植体嫩茎的芽分化能力比嫩叶和根的强。嫩茎作为组培耐盐种质筛选的外植体是最适宜的。②在５．０ｇ·Ｌ￣（－１）盐胁迫条件下，无性系ＮＬ－８０１０５和ＮＬ－８０１０６的芽分化能力比无性系ＮＬ－８０３０３的强，以无性系ＮＬ－８０１０５为最强。③采用ＭＳ为基本培养基，附加６－ＢＡ０．４０ｍｇ·Ｌ－１、ＮＡＡ０．０２ｍｇ·Ｌ￣（－１）的浓度组合筛选耐盐再生小植株较为适宜。④经过逐渐提高盐浓度的分阶段筛选法，获得了能耐质量浓度为１０．０ｇ·Ｌ－１氯化钠的愈伤组织系，耐１０．０ｇ·Ｌ￣（－１）氟化钠和１０．０ｇ·Ｌ￣（－１）海水盐的无性系ＮＬ－８０１０５和ＮＬ－８０１０６不定芽若干以及耐７．５ｇ·Ｌ￣（－１）氯化钠的无性系ＮＬ－８０１０５再生小植株２１株和天性系ＮＬ－８０１０６再生小植株２株。The Selection of salt to lerance plantlet regeneration of three poplar clones,namely poplar NL-80105, poplar NL-80106, and poplar NL-80303, in tissucs was studied,The rcsults were as follows1)On MS medium with 5.0g·L￣(-1) NaCl, the most shoots formed on young stem explants among threo explants,amd more shoots developed from clone NL-80105 and NL-80106 than from clone NL-80303.(2) More salt tolerance shoots formed from the explants treated with EMS or γ ray than from those untreated.(3)MS medium with 6-BA 0.40 mg·kg￣ (-1) and NAA 0.02 mg·kg￣(-1) was suitable for selection of salt tolerance poplar regenerated plantlet.(4)By elimination series under higher salt density,after all, the calli tolerance to 10.0g· kg￣(-1) NaCl , the shoots tolerance to 10.0g·L￣(-1) Nacl or sea salt and 23 regenerated plantlets resistant 7.5g·L￣(-1) Nacl,21 from clone NL-80105 and 2 from clone NL-80106,were obtained.

关 键 词：杨属 无性系 组织培养 耐盐性 种质资源 

分 类 号：S792.110.4[农业科学—林木遗传育种]