MTT法评价国产碳纤维桩材料的细胞毒性  

Biological evaluation of domestic carbon fiber-reinforced post materials

作　　者：郑晓雁[1] 张振庭[1] 方玉[2] 

机构地区：[1]首都医科大学口腔医学院修复科,北京100050 [2]中国药品生物制品检定所

出　　处：《北京口腔医学》2006年第3期189-191,共3页Beijing Journal of Stomatology

基　　金：国家自然科学基金(50373047);北京市自然科学基金(7992019)资助项目

摘　　要：目的研究四种国产碳纤维桩材料的细胞毒性。方法采用MTT比色法检测碳纤维桩材料对小鼠成纤维细胞L-929活性与增殖的影响,计算相对增殖率RGR,评价其细胞毒性。结果四种国产碳纤维桩材料的RGR值均>75%,且RGR值依次增加,细胞毒性均为1级。结论四种国产碳纤维桩材料均无明显细胞毒性,其中一种材料的细胞相容性最好。Objective To evaluate the cytotoxicity of four types of native carbon fiber-reinforced post materials （ polyimide, PI）. Methods The viability and proliferation of L-929 cells were tested by MTT assay. Relative growth rates of cells were calculated and the cytotoxicities were graded by generally accepted standard. Results Relative growth rates of PI1-4 were more than 75% and increased gradually. The cytotoxicity of PI1-4 was grade 1. Conclusion The four types of native carbon fiber-reinforced post materials have no evident cytotoxicity, and PI4 has the best cytocompatibility.

关 键 词：碳纤维桩 生物学评价 细胞毒性 

分 类 号：R114[医药卫生—卫生毒理学]