转Bt基因抗虫玉米对亚洲玉米螟幼虫几种主要酶系活性的影响  

Effects of transgenic Bt corn expressing Cry1Ab toxin on activities of some enzymes in larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae)

作　　者：徐艳聆[1] 王振营[1] 何康来[1] 白树雄[1] 

机构地区：[1]中国农业科学院植物保护研究所

出　　处：《昆虫学报》2006年第4期562-567,共6页Acta Entomologica Sinica

基　　金：国家重点基础研究发展规划"973"项目(2001CB109004);国家自然科学基金项目(30370967)

摘　　要：研究了表达Cry1Ab杀虫蛋白的转Bt基因抗虫玉米对亚洲玉米螟Ostriniafurnacalis(Guen啨e)幼虫解毒酶、保护酶和中肠蛋白酶活性的影响,测定比较了取食转Bt基因玉米后幼虫体内α乙酸萘酯酶、乙酰胆碱酯酶、谷胱甘肽S转移酶、过氧化氢酶、超氧化物歧化酶、中肠总蛋白酶、类胰蛋白酶和类胰凝乳蛋白酶的活力。结果表明,取食转Bt基因玉米48h后亚洲玉米螟幼虫体内的α乙酸萘酯酶、谷胱甘肽S转移酶活力明显低于对照;而乙酰胆碱酯酶活力显著高于对照,在取食48h、60h和72h的活力分别是对照的2.00、1.50和2.50倍。保护酶系、中肠总蛋白酶、弱碱性类胰蛋白酶和类胰凝乳蛋白酶的活性在取食48h后明显受到抑制;但强碱性类胰蛋白酶的活性显著高于对照,取食48h、60h和72h的活力分别是对照的4.00、1.67和1.33倍。乙酰胆碱酯酶和强碱性类胰蛋白酶可能与亚洲玉米螟对Bt的抗性有关。The activities of three detoxification enzymes （α-naphthyl acetate esterase, acetylcholinesterase and glutathione-S-transferase）, two protective enzymes （superoxide dismutase and eatalase） and midgut proteases （total protease, trypsin-like enzyme and chymotrypsin-like enzyme） in 3rd instar larvae of the Asian corn borer, Ostrinia fumacalis after feeding on Bt-transgenic corn were evaluated in the laboratory. The results showed that the activities of α-naphthylacetate esterase, glutathione-S-transferase in the larvae fed on Bt corn leaf for 48 h were greatly lower than that of the control fed on the non-transgenic com. However, the acetylcholinesterase activity of the larvae fed on Bt corn for 48 h, 60 h and 72 h increased markedly, being 2.00-, 1.50- and 2.50-fold higher than that of the control, respectively. The activities of protective enzymes, total protease, weak trypsin-like enzyme and chymotrypsin-like enzyme in the larvae fed on Bt corn for 48 h were also significantly lower than that of the control. However, the activities of the active alkaline trypsin-like enzyme were obviously higher than those of the control. The active alkaline trypsin-like enzyme activities of the larvae fed on Bt corn for 48 h, 60 h and 72 h were 4.00-, 1.67- and 1.33-fold higher than that of the control. The results suggest that acetylcholinesterase and active alkaline trypsin-like enzyme may be associated with Asian corn borer resistance to Bt.

关 键 词：亚洲玉米螟 CrylAb杀虫蛋白 转BT基因玉米 解毒酶 保护酶 蛋白酶 

分 类 号：Q965[生物学—昆虫学]