甘肃文县婴儿利什曼原虫无症状感染犬的检测  

Survey on the Leishmania infantum asymptomatic infection in dogs in Wenxian county of Gansu province

作　　者：汪俊云[1] 陈生邦[2] 高春花[1] 金长发[1] 冯宇[2] 张丑吉[2] 何慧娴[2] 杨成明[2] 杨涛 包意芳[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,上海200025 [2]甘肃省疾病预防控制中心寄生虫病防治科

出　　处：《中国人兽共患病学报》2006年第8期734-737,共4页Chinese Journal of Zoonoses

基　　金：WHO/TSA基金资助课题(No.1079946)

摘　　要：目的评价PCR法、ELISA法和试条法检测我国婴儿利什曼原虫无症状感染犬的潜能。方法用两组PCR引物RV1-RV2和K13A-K13B检测动物源型黑热病疫区健康犬静脉血和骨髓中利什曼原虫特异DNA,以利什曼原虫可溶性抗原为包被抗原的ELISA法和rk39-dipstick试条法分别检测利什曼原虫特异抗体,并比较各种检测方法的敏感性差异。结果PCR法检测抗凝静脉血和骨髓的阳性率分别为50.63%(40/79)和69.62%(55/79),两种样本总检出率为77.21%(61/79);ELISA法检测的阳性率为22.22%(16/72),而rk39-dipstick试条检测的阳性率为33.33%(19/57)。结论我国动物源性黑热病疫区利什曼原虫无症状感染犬的比例相当高,以骨髓为样本的PCR检测法为较精确的犬无症状感染检测方法。To evaluate the potential of PCR, ELISA and rk39-dipstick for the detection of Leishmania infantum asymptomatie infection in dogs in western China, samples from healthy dogs in Wenxian country of Gansu province, the endemic area of zoonotic visceral leishmaniasis, were collected and subjected to be tested for the leishmania-specific DNA in venous blood and bone marrow of the healthy dogs in this area by means of PCR assay with two pairs of primers RV1-RV2 and K13A-K13B. Meanwhile, the Leishmania-specific antibodies were detected by ELISA coated with soluble antigen and the rk39-dipstick. It was found that the positive detection rates of PCR in venous blood and bone marrow samples were 50.63% （40/47） and 69. 62% （55/79）, with a total detection rate of 77.21% （61/79）% those of ELISA and rk39-dipstick were 22.22% （16/72） and 33.33% （19/57）. It is evident that the prevalence of the asmptomatic infection with L. infantum in dogs is rather high, and the PCR assay for bone marrow specimens is proved to be a reliable method to detect the asymptomatic infection in dogs.

关 键 词：婴儿利什曼原虫 无症状感染 PCR ELISA rk39-dipstick 

分 类 号：R382.2[医药卫生—医学寄生虫学]