吉林林区动物莱姆病螺旋体感染的调查研究  

Investigation on Borrelia burgdorferi infection in ticks and animal from a forest area of Jilin province

作　　者：黄海楠[1] 丁壮[2] 何静[1] 吴晓明[1] 蒋宝贵[1] 高燕[1] 赵秋敏[1] 王玉福[3] 曹务春[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [2]吉林大学畜牧兽医学院重要病原与疫病研究室 [3]吉林省畜牧兽医总站

出　　处：《中国人兽共患病学报》2006年第8期785-788,共4页Chinese Journal of Zoonoses

基　　金：国家科技攻关计划课题资助项目(2003BA712A05-01);全军医学科学技术研究重大专项(06D006-1)

摘　　要：目的了解吉林珲春林区的蜱、野鼠及牛、绵羊感染莱姆病的情况。方法对蜱和野鼠进行莱姆病螺旋体的PCR扩增,阳性标本RFLP分型,应用间接免疫荧光法检测家畜血清中的IgG抗体。结果PCR检测全沟硬蜱和森林革蜱的带菌率为36.0%和30.9%;在五种鼠中检测到莱姆病螺旋体的特异性片段,带菌率分别为14.0%、8.3%、13.0%、25.0%和33.3%。阳性标本RFLP分型结果属于B.garinii和B.afzelii型;牛、羊血清学检测阳性率为27.5%和31.5%。结论野鼠、蜱和牛羊中都检测到伯氏疏螺旋体的感染,证实吉林珲春林区存在莱姆病的自然疫源地。To study the Borrelia burgdorferi sensu lato （B. b. s. l） infection in ticks, rodents and livestocks at forestry of Hunchun, Jilin province. PCR was used to detect 5S-23S rRNA intergenic region of B. b. s. l in ticks and rodents, and the restriction fragment length polymorphism （RFLP） was used to type the positive samples. IFA was used to detect the IgG antibody in sera of livestocks. A total of 100 I. persulcatus ticks and 327 D. silvarum ticks collected in forestry of Hunchun were investigated. The infection rates in I. persulcatus ticks and D. silvarum ticks were 36.0% and 30.9% respectively. The specific DNA fragments of B. b. s. 1. had been detected in Apodemus speciosus, A. agrarius, Clethrionomys rufocanus, Cricetulus triton and Eutamias sibiricus, with infective rates of 14.0%, 8. 3%, 13.0%, 25.0% and 33.3%, respectively. The results of RFLP for genotyping of the positive samples in ticks demonstrated that they were proved to be B. garinii and B. afzelii genotypes. About 200 sera of cattle and 203 sera of sheep were detected by IFA. The positive rates of serological detection in cattle and sheep were 27.5% and 31.5% respectively. The infection of B. b. s. l. had been detected among vectors, rodents and livestocks, all the three taches demonstrated that Hunchun forestry of Jilin province are natural foci of Lyme disease.

关 键 词：伯氏疏螺旋体 蜱 野鼠 家畜 

分 类 号：R377[医药卫生—病原生物学]