重组鸡痘病毒表达的鸡IL-1β和IL-2以及cMGF对新城疫LaSota疫苗免疫效果的影响  

Effects of chicken IL-1β,IL-2 and cMGF expressed by the recombinant fowlpox viruses on immunization with NDV LaSota vaccine

作　　者：邵卫星[1] 卢建红[1] 彭大新[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室

出　　处：《中国兽医科学》2006年第8期617-620,共4页Chinese Veterinary Science

基　　金：江苏省自然科学基金项目(BK2001415)

摘　　要：将128只10日龄SPF鸡随机分成8组,每只免疫1 PD_(50)的新城疫LaSota疫苗；同时Ⅰ～Ⅵ组鸡分别皮下注射不同剂量的重组鸡痘病毒rFPV-IL-1β、rFPV-IL-2或cMGF,而Ⅶ组和Ⅷ组鸡分别皮下注射鸡痘病毒wt-FPV和PBS作为阴性对照和空白对照。结果,重组鸡痘病毒表达的IL-1β、IL-2或cMGF可以消除鸡痘病毒在免疫后第7d对雏鸡体重增长的影响；一定剂量的IL-1β和IL-2可使鸡脾中CD4^+T细胞在免疫后第7d和CD8^+T细胞在免疫后第14d的总体水平提高；免疫第21d新城疫F48E8强毒攻毒后,一定剂量的IL-1β和IL-2能提高免疫保护率,而cMGF却无此作用。结果表明,重组鸡痘病毒表达的IL-1β和IL-2在一定剂量下可增强新城疫LaSota疫苗的免疫效果。The 128 10-days-old SPF chickens were divided randomly into 8 groups with 16 each, and immunized with 1 PD50 of the LoSota vaccine per chicken. After immunization, Group Ⅰ to Ⅵ chickens were inoculated hypodermicly with 1×10^3 and 1×10^5 PFU of rFPV-IL-1β 1×10^3 and 1×10^5 PFU of rFPV-IL-2, and 1×10^3 and 1×10^5 PFU of rFPV-cMGF（chicken myelomonocytic growth factor） respectively, Group Ⅶ with 1×10^5 PFU of wt-FPV and Group Ⅷ with 0.1 mL of PBS solution, per chicken. In results, IL-1β, IL-2 and cMGF expressed by rFPV protected significantly against body weight loss induced by rFPV on day 7 post-immunization and increased significantly levels of CD4^＋ T cells on day 7 and levels of CD8^＋ T cells in spleen of the chickens on day 14 post-immunization. After challenge with NDV F48E8 on day 21 post-immunization, chickens inoculated with 1×10^3 PFU of rFPV-IL-1β and rFPV-IL-2 each were significantly protected against death in comparison of chickens received 1×10^5 PFU of the parental virus wt-FPV each. The results showed that a certain amount of rFPV-expressed IL-1β and IL-2 as adjuvant can promote cell-mediated immunity and improve the protection efficacy of the LoSota vaccine.

关 键 词：鸡痘病毒 白细胞介素-1β 白细胞介素-2 鸡髓细胞生长因子 新城疫LaSota疫苗 免疫效果 

分 类 号：S852.659.1[农业科学—基础兽医学]