贵州四个民族人群线粒体DNA RegionⅤ的遗传多态性  

Polymorphism of Mitochondrial DNA Region Ⅴ in Four Ethnic Populations from Guizhou

作　　者：李彬彬[1] 钟复光[2] 易红生[2] 王先然 李良芳 王丽兰 齐晓岚[2] 吴立甫[2] 

机构地区：[1]广东医学院病理生理学教研室,湛江524023 [2]贵阳医学院医学科学研究所,贵阳550004 [3]贵州省天柱县人民医院,天柱556700 [4]贵州省赫章县人民医院,赫章553200 [5]贵州省务川县人民医院,务川564300

出　　处：《人类学学报》2006年第3期258-262,共5页Acta Anthropologica Sinica

基　　金：国家自然科学基金资助项目(39560035)

摘　　要：目的研究世居贵州的侗族、仡佬族、土家族和彝族人群线粒体DNA RegionⅤ的遗传多态性。方法采用PCR-PAGE和克隆测序法对4个群体108份样本的mtDNA RegionⅤ进行序列分析。结果只检测到标准型和短型(即9-bp缺失)两种多态。贵州四个民族人群的平均9-bp缺失频率为22.2%,在侗族、仡佬族、土家族和彝族人群中依次为32.1%、22.6%、17.2%和15.0%。结论贵州四个民族mtDNA 9-bp缺失频率均较高,这与其地域分布相一致;贵州彝族和土家族显示了相似的缺失频率,提示两者可能有共同的祖先。Objective To study the polymorphism of mitochondrial DNA（mtDNA） regionV in Dong, Gelao,Tujia and Yi ethnic population from Guizhou. Method The regionV sequences in total of 108 samples from four ethnic populations were analyzed by polymerase chain reaction and polyacrylamide gel electrophoresis （PCR-PAGE） and clonal sequencing. Result Only two kinds of polymorphism were found in these populations. One was standard patter, the other was short patter（ namely 9-bp deletion ）. The average frequency of 9-bp deletion in four ethnic populations from Guizhou was 22.2%, and the frequencies of 9-bp deletion in Dong,Gelao,Tujia and Yi population were 32.1%, 22.6% ,17.2%and 15.0% respectively. Conclusion The frequencies of 9-bp deletion are higher in four ethnic populations from Guizhou, which is consistent with their regional distribution, the frequencies of deletion in Tujia and Yi population are similar, this suggests they might originate from a common ancestry.

关 键 词：贵州民族人群 线粒体DNA 遗传多态性 

分 类 号：Q987[生物学—遗传学]