CD11b/DNA双参数流式细胞术检测HL-60细胞分化的细胞周期  

ANALYSIS OF CELL CYCLE IN THE DIFFERENTIATION OF HL-60 CELLS BY CD11b/DNA MULTIPARAMETER FLOW CYTOMETRY

作　　者：文若剑[1] 谢大兴[1] 张鹏[1] 陶德定[1] 龚建平[1] 余从年[2] 

机构地区：[1]华中科技大学同济医学院同济医院分子医学中心,武汉430030 [2]华中科技大学同济医学院细胞生物学教研室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2006年第4期427-432,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(39730270);973肿瘤计划(G1998051212);卫生部临床重点学科资助项目(200112537)

摘　　要：目的采用双参数流式细胞术研究全反式维甲酸(alltransretinoidacid,ATRA)诱导人类急性早幼粒白血病细胞HL-60细胞分化的细胞周期。方法HL-60细胞经分化诱导剂ATRA(终浓度为1μmol/L)诱导不同时间点后,利用CD11b/DNA双参数流式细胞术同时检测分化细胞表面抗原CD11b的表达及分化细胞DNA含量。结果HL-60细胞经ATRA诱导后,细胞表面分化抗原CD11b表达明显升高,细胞阻滞于G0/G1期,且CD11b阳性细胞主要位于G0/G1期。结论CD11b/DNA双参数流式细胞术能简便,快速,直观地检测细胞分化的细胞周期。Objective To investigate the cell cycle specificity in the differentiation of human promyelocytic leukemia cells HL-60 by multiparameter flow cytometry. Methods Being induced by ATRA （final concentration is μmol/L） at different times, the cellular surface antigen CD11b expression and DNA content were dctected simultaneously by multiparameter flow cytometry. Results After the treatment with ATRA, Cells were arrested at the G0/G1 phase, and cell surface differentiation antigen CD11b was significantly increased. Most of the differentiated cells had the DNA content at the G0/G1 phase. Conclusion The analysis of the cell cycle in the differentiation of HL-60 cells by CD11b/DNA multiparameter flow cytometry is simple, fast and readily observable.

关 键 词：全反式维甲酸 HL-60细胞 细胞分化 细胞周期 双参数流式细胞术 

分 类 号：Q461[生物学—生理学]